Effect of Hypothermia on Kinetic Characteristics of Lactate Dehydrogenase in Rat Brain under Conditions of Global Ischemia and Reperfusion
- Authors: Khalilov R.A.1, Dzhafarova A.M.1, Khizrieva S.I.1
- 
							Affiliations: 
							- Department of Biochemistry and Biophysics, Dagestan State University
 
- Issue: Vol 163, No 3 (2017)
- Pages: 334-337
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238865
- DOI: https://doi.org/10.1007/s10517-017-3797-8
- ID: 238865
Cite item
Abstract
We studied activity and kinetic characteristics of lactate dehydrogenase (LDH) in rat brain under conditions of incomplete global ischemia followed by reperfusion against the background of mild hypothermia. It was found that hypothermia leads to a decrease in LDH activity in the ischemic brain; the maximum velocity of the enzyme-catalyzed activity decreased and Michaelis constant increased, due to which the efficiency of catalysis decreased to the level observed in control rats. Ischemia against the background of hypothermia was accompanied by a decrease in the inhibition constant and narrowing of effective pyruvate concentration range. Blood flow resumption in the ischemic brain against the background of mild hypothermia led to an increase in LDH activity, the maximum reaction velocity increased, and Michaelis constant decreased, which lead to a significant increase in the efficiency of catalysis. This was accompanied by an increase in enzyme inhibition constant and a shift of the optimum on the concentration curve towards lower pyruvate concentrations.
About the authors
R. A. Khalilov
Department of Biochemistry and Biophysics, Dagestan State University
														Email: albina19764@mail.ru
				                					                																			                												                	Russian Federation, 							Republic of Dagestan, Makhachkala						
A. M. Dzhafarova
Department of Biochemistry and Biophysics, Dagestan State University
							Author for correspondence.
							Email: albina19764@mail.ru
				                					                																			                												                	Russian Federation, 							Republic of Dagestan, Makhachkala						
S. I. Khizrieva
Department of Biochemistry and Biophysics, Dagestan State University
														Email: albina19764@mail.ru
				                					                																			                												                	Russian Federation, 							Republic of Dagestan, Makhachkala						
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