Email this article Internalization of Vectorized Liposomes Loaded with Plasmid DNA in C6 Glioma Cells
- Authors: Mel’nikov P.A.1,2, Baklaushev V.P.2, Gabashvili A.N.1, Nukolova N.V.1,3, Kuznetsov I.I.4, Cherepanov S.A.1, Koshkin F.A.1, Leopol’d A.V.1, Chekhonin V.P.1,3
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Affiliations:
- Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Federal Clinical Research Centre, Federal Medical-Biological Agency
- Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
- Department of Chemical Enzymology, Faculty of Biology, M. V. Lomonosov Moscow State University
- Issue: Vol 163, No 1 (2017)
- Pages: 114-122
- Section: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/238700
- DOI: https://doi.org/10.1007/s10517-017-3750-x
- ID: 238700
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Abstract
We studied internalization of vector nanocarriers loaded with plasmid DNA into C6 glioma cells. For improving selectivity of plasmid delivery, the liposomes were conjugated with monoclonal antibodies to VEGF and its receptor VEGFR2. Flow cytofluorometry and laser scanning confocal microscopy showed more intensive (more than 2-fold) internalization and accumulation of antibody-vectorized liposomes in C6 glioma cells in comparison with the control (liposomes conjugated with non-specific antibodies and non-vectorized liposomes). Using quantitative analysis of fluorescent signal, we showed that cationic immunoliposomes significantly more effective delivered pCop-Green-N plasmid DNA and ensured effective transfection of C6 glioma cells.
About the authors
P. A. Mel’nikov
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Federal Clinical Research Centre, Federal Medical-Biological Agency
Author for correspondence.
Email: proximopm@gmail.com
Russian Federation, Moscow; Moscow
V. P. Baklaushev
Federal Clinical Research Centre, Federal Medical-Biological Agency
Email: proximopm@gmail.com
Russian Federation, Moscow
A. N. Gabashvili
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow
N. V. Nukolova
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
Email: proximopm@gmail.com
Russian Federation, Moscow; Moscow
I. I. Kuznetsov
Department of Chemical Enzymology, Faculty of Biology, M. V. Lomonosov Moscow State University
Email: proximopm@gmail.com
Russian Federation, Moscow
S. A. Cherepanov
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow
F. A. Koshkin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow
A. V. Leopol’d
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: proximopm@gmail.com
Russian Federation, Moscow
V. P. Chekhonin
Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University
Email: proximopm@gmail.com
Russian Federation, Moscow; Moscow
