Stimulation of Diethylnitrosamine Metabolism Reduces Its General Toxic and Hepatocarcinogenic Effects
- Authors: Il’nitskaya S.I.1, Kaledin V.I.1, Bogdanova L.A.2, Morozkova T.S.2, Kapustina V.I.2, Perepechaeva M.L.3, Grishanova A.Y.3
-
Affiliations:
- Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
- Institute of Molecular Pathology and Pathomorphology
- Research Institute of Molecular Biology and Biophysics
- Issue: Vol 162, No 1 (2016)
- Pages: 98-101
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237949
- DOI: https://doi.org/10.1007/s10517-016-3555-3
- ID: 237949
Cite item
Abstract
The general toxic and hepatocarcinogenic effects of diethylnitrosamine after stimulation of its metabolism with 1,4-bis[2-(3,5-dichloropyridyloxy)]-benzene (TCPOBOP) were studied. The hydroxylating activity of liver microsomes of C57Bl/6Mv mice towards p-nitrophenol increased more than 4-fold 3 days after injection of TCPOBOP. Injection of diethylnitrosamine 3 days after TCPOBOP caused a lesser body weight loss and decrease of food consumption in C57Bl/6Mv mice than in response to diethylnitrosamine without preinduction. Injection of diethylnitrosamine to suckling ICR mice after TCPOBOP induction of cytochrome P450 2e1 activity led to development of 2-fold lesser number of tumors and pretumorous nodes in the liver in comparison with animals injected with diethylnitrosamine without induction. These data indicated that metabolism stimulation reduced the general toxic and hepatocarcinogenic effects of diethylnitrosamine.
About the authors
S. I. Il’nitskaya
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Author for correspondence.
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
V. I. Kaledin
Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
L. A. Bogdanova
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
T. S. Morozkova
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
V. I. Kapustina
Institute of Molecular Pathology and Pathomorphology
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
M. L. Perepechaeva
Research Institute of Molecular Biology and Biophysics
Email: pathol@inbox.ru
Russian Federation, Novosibirsk
A. Yu. Grishanova
Research Institute of Molecular Biology and Biophysics
Email: pathol@inbox.ru
Russian Federation, Novosibirsk