Preparation of Viscumin-Ferromagnetic Particles Conjugate and Study of Its Internalization by Human Glioblastoma A172 Cells
- Authors: Khutornenko A.A.1, Gerasimov V.M.1, Sakharov D.A.1
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Affiliations:
- BioClinicum Research and Technology Center
- Issue: Vol 160, No 6 (2016)
- Pages: 823-826
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236975
- DOI: https://doi.org/10.1007/s10517-016-3319-0
- ID: 236975
Cite item
Abstract
Magnetite particles modified by polyethylene glycol with a molecular weight of 3 kDa and hydrodynamic diameter of ~60 nm were used. Plant lectin viscumin covalently immobilized on these nanoparticles retained its binding activity. Immunochemical characteristics of conjugated viscumin were evaluated using monoclonal antibodies. The resultant conjugate with a hydrodynamic diameter of 70 nm was used for studies of binding and internalization by target cells. Binding of viscumin and its conjugate was determined by receptors containing terminal galactose, while intracellular distribution varied. The model system presented in this study can be used for creation of drugs for target therapy.
About the authors
A. A. Khutornenko
BioClinicum Research and Technology Center
Email: d.sakharov@bioclinicum.com
Russian Federation, Moscow
V. M. Gerasimov
BioClinicum Research and Technology Center
Email: d.sakharov@bioclinicum.com
Russian Federation, Moscow
D. A. Sakharov
BioClinicum Research and Technology Center
Author for correspondence.
Email: d.sakharov@bioclinicum.com
Russian Federation, Moscow
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