Oxidized Dextran Enhances Alternative Activation of Macrophages in Mice of Opposite Lines
- Authors: Chechushkov A.V.1, Kozhin P.M.1, Zaitseva N.S.1, Lemza A.E.1, Men’shchikova E.B.1, Troitskii A.V.1, Shkurupy V.A.1,2
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Affiliations:
- Research Institute of Experimental and Clinical Medicine
- Novosibirsk State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 6 (2016)
- Pages: 783-786
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236940
- DOI: https://doi.org/10.1007/s10517-016-3309-2
- ID: 236940
Cite item
Abstract
Differences in peritoneal macrophage polarization in mice of opposite lines CBA and C57Bl/6 and the effects of 60 kDa oxidized dextran were studied. Macrophages of C57Bl/6 mice demonstrated a phenotype close to M1, with increasing expression of CD86 costimulatory molecule and unchanged CD206 expression in response to activation. Macrophages of CBA mice demonstrated higher plasticity in response to activating agents; expression of the markers increased irrespectively on stimulated receptor (TLR-4 or mannose receptor) and both CD86 (classical activation) and CD206 (alternative activation) increased. Macrophage response to addition of oxidized dextran (60 kDa) to the culture medium could be characterized as potentiation of their alternative activation: expression of CD86 in CBA mice in response to LPS and LPS+IL-4 and in C57Bl/6 mice in response to IFN-γ and LPS+IFN-γ decreased, while expression of CD206 by intact macrophages of CBA mice and by macrophages stimulated by IFN-γ and IL-4 increased under the effect of 60 kDa oxidized dextran.
About the authors
A. V. Chechushkov
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
P. M. Kozhin
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
N. S. Zaitseva
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
A. E. Lemza
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
E. B. Men’shchikova
Research Institute of Experimental and Clinical Medicine
Author for correspondence.
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
A. V. Troitskii
Research Institute of Experimental and Clinical Medicine
Email: lemen@centercem.ru
Russian Federation, Novosibirsk
V. A. Shkurupy
Research Institute of Experimental and Clinical Medicine; Novosibirsk State Medical University, Ministry of Health of the Russian Federation
Email: lemen@centercem.ru
Russian Federation, Novosibirsk; Novosibirsk