Email this article Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles
- Authors: Romanov Y.A.1,2, Balashova E.E.1,2, Volgina N.E.3, Kabaeva N.V.1, Dugina T.N.2, Sukhikh G.T.3
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Affiliations:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Umbilical Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 4 (2016)
- Pages: 571-574
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236796
- DOI: https://doi.org/10.1007/s10517-016-3220-x
- ID: 236796
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Abstract
We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
About the authors
Yu. A. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Umbilical Cord Blood Bank
Author for correspondence.
Email: romanov@cardio.ru
Russian Federation, Moscow; Moscow
E. E. Balashova
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Umbilical Cord Blood Bank
Email: romanov@cardio.ru
Russian Federation, Moscow; Moscow
N. E. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
N. V. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
T. N. Dugina
CryoCenter Umbilical Cord Blood Bank
Email: romanov@cardio.ru
Russian Federation, Moscow
G. T. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
