Email this article Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue
- Authors: Romanov Y.A.1,2, Balashova E.E.1,3, Volgina N.E.3, Kabaeva N.V.1, Dugina T.N.3, Sukhikh G.T.3
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Affiliations:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Umbilical Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Issue: Vol 160, No 4 (2016)
- Pages: 530-534
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/236771
- DOI: https://doi.org/10.1007/s10517-016-3213-9
- ID: 236771
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Abstract
Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies.
About the authors
Yu. A. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Umbilical Cord Blood Bank
Author for correspondence.
Email: romanov@cardio.ru
Russian Federation, Moscow; Moscow
E. E. Balashova
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow; Moscow
N. E. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
N. V. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
T. N. Dugina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
G. T. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Russian Federation, Moscow
