DEVELOPMENT OF FEMALE REPRODUCTIVE STRUCTURES IN BOECHERA (BRASSICACEAE) SPECIES UNDER SEXUAL AND APOMICTIC REPRODUCTIVE MODES

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Abstract

The results of comparative embryological study of the two species from the genus Boechera (B. falcata and B. stricta), which are characterized by a sexual mode of reproduction, and the apomictic M4B accession, an ancient natural hybrid of B. stricta × B. retrofracta, are presented. A detailed description of the ovule development of the studied plants is described. A significant similarity of the ovule development has been shown; a general plan of the ovules structure is ortho-campylotropic, bitegmal, medionucellate. The development of the embryo sac in B. falcata and B. stricta corresponds to the monosporic Polygonum type; in the ovules of the M4B accession, an apomictic unreduced female gametophyte is predominantly formed as a result of Taraxacum-type diplospory. However, in this accession apomixis is facultative, since a small amount of the ovules in the ovary forms a reduced embryo sac with participation of meiosis, but unlike B. falcata and B. stricta, after the second meiotic division, a triad of megaspores is formed, of which the chalazal one is functional. In the ovules of the apomictic M4B accession, underdevelopment of the inner integument was often observed, which, apparently, correlates with arrest of the megasporocyte and embryo sac development, since destruction was often observed in ovules with a short inner integument. This abnormality is likely due to the hybrid nature of the M4B accession rather than apomixis. In this accession, the embryo is formed parthenogenetically, but its development requires endosperm, which apparently develops by pseudogamy, only after triple fusion (fertilization of the fused polar nuclei by sperm). In the absence of endosperm, the embryo perishes, and seed development is arrested.

About the authors

G. Yu. Vinogradova

Komarov Botanical Institute of RAS

Author for correspondence.
Email: vinogradova-galina@binran.ru
Russia, 197022, St. Petersburg, Prof. Popova Str., 2

N. V. Sinelnikova

Institute of Biological Problems of the North, Far Eastern Branch of RAS

Author for correspondence.
Email: meks_mag@mail.ru
Russia, 685000, Magadan, Portovaya Str., 18

K. M. Taşkin

Çanakkale Onsekiz Mart University

Author for correspondence.
Email: kmtaskin@comu.edu.tr
Turkey, 17100, Çanakkale

V. B. Brukhin

Komarov Botanical Institute of RAS

Author for correspondence.
Email: vbrukhin@gmail.com
Russia, 197022, St. Petersburg, Prof. Popova Str., 2

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