Email this article Characterization of an Acellular Dermal Matrix Obtained by Different Detergent-Enzymatic Methods
- Authors: Sarbaeva N.N.1, Milyakova M.N.1, Rossinskaya V.V.1, Gribkova O.V.1, Dolgushkin D.A.1
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Affiliations:
- Samara State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 51, No 3 (2017)
- Pages: 166-169
- Section: Article
- URL: https://journals.rcsi.science/0006-3398/article/view/235030
- DOI: https://doi.org/10.1007/s10527-017-9707-6
- ID: 235030
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Abstract
Acellular dermal matrixes (ADM) for reconstructive surgery were made by treating skin samples from rats and humans with sodium dodecylsulfate (SDS), sodium deoxycholate (DOC), saponin, and DNase. A mixture of SDS and DOC removed cell residues effectively, though the ADM had cytotoxic properties. After treatment with 2% DOC, there was no cytotoxic effect and the resulting material contained 27-202 ng/mg dry weight DNA and from 0.07 to 2.6% fat, depending on the source of the samples.
About the authors
N. N. Sarbaeva
Samara State Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: sarbaeva@gmail.com
Russian Federation, Samara
M. N. Milyakova
Samara State Medical University, Ministry of Health of the Russian Federation
Email: sarbaeva@gmail.com
Russian Federation, Samara
V. V. Rossinskaya
Samara State Medical University, Ministry of Health of the Russian Federation
Email: sarbaeva@gmail.com
Russian Federation, Samara
O. V. Gribkova
Samara State Medical University, Ministry of Health of the Russian Federation
Email: sarbaeva@gmail.com
Russian Federation, Samara
D. A. Dolgushkin
Samara State Medical University, Ministry of Health of the Russian Federation
Email: sarbaeva@gmail.com
Russian Federation, Samara
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