Lipase and esterase activity in soils from ancient burial vessels
- 作者: Kashirskaya N.1, Chernysheva E.1, Udaltsov S.1, Plekhanova L.1
-
隶属关系:
- Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences
- 期: 卷 68, 编号 6 (2023)
- 页面: 1200-1209
- 栏目: Articles
- URL: https://journals.rcsi.science/0006-3029/article/view/249693
- DOI: https://doi.org/10.31857/S000630292306011X
- EDN: https://elibrary.ru/ROSYVA
- ID: 249693
如何引用文章
详细
The activity of lipolytic enzymes was assessed using the microplate method using heteromolecular exchange in soils from the bottom of burial vessels, followed by cluster analysis. Groups with a contrasting ratio of lipases C12 - C18 and esterases C4 - C8 were identified in a sample that included 57 burial pots aged two to three thousand years. The total lipolytic activity of soil samples from dishes with the presumptive initial presence of fat-containing food products was above 6% of the maximum value within the sample. Inhibition of esterases in the presence of high concentrations of fatty acids is reflected in the extremely low relative values of esterase activity of the soil microbial community against the background of maximum activity levels of C18 and C12 lipases in two groups of pots. In three groups, esterase activity was observed against the background of high relative activity of C16 lipases, average relative activity of C18 lipases and extremely low activity of true lipases. In the group of pots with high total lipolytic activity (with the exception of certain extreme values of this indicator), the proportion of true lipase activity was 1.7-1.8 times higher compared to the proportion of esterase activity. The grouping of burial vessels by the ratio of lipase and esterase activity reflects the characteristics of the funeral food, while the absolute values of enzyme activity among selected vessels with the highest total lipolytic activity are grouped according to the type of burial.
作者简介
N. Kashirskaya
Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of SciencesPushchino, Moscow region, Russia
E. Chernysheva
Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of SciencesPushchino, Moscow region, Russia
S. Udaltsov
Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of SciencesPushchino, Moscow region, Russia
L. Plekhanova
Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences
Email: dianthus1@rambler.ru
Pushchino, Moscow region, Russia
参考
- R. P. Evershed, Archaeometry, 50, 895 (2008). doi: 10.1111/j.1475-4754.2008.00446.x
- L. J. Cramp, I. D. Bull, E. Casanova, et al., in Lipids in Archaeology. Handbook of Archaeological Sciences (2023)., V. 1, p. 529
- A. Irto, G. Micalizzi, C. Bretti, et al., Molecules, 27 (11), 3451 (2022).
- M. S. Copley, F. A. Hansel, K. Sadr, and R. P. Evershed, South Afr. J. Sci., 100 (5), 279 (2004).
- R. P. Evershed, S. N. Dudd, M. S. Copley, et al., Accounts Chem. Res., 35 (8), 660 (2002).
- S. Hammann, D. J. Scurr, M. R. Alexander, and L. J. Cramp, Proc. Natl. Acad. Sci., 117 (26), 14688 (2020).
- M. P. Colombini, F. Modugno, and E. Ribechini, J. Mass Spectrometry, 40 (7), 890 (2005).
- M. Bondetti, et al., Archaeometry, 63 (3), 594 (2021).
- M. S. Copley, P. J. Rose, A. Clapham, et al., Proc. Roy. Soc. Lond. Ser. B: Biol. Sci., 268 (1467), 593 (2001).
- R. Sacchi, et al., Science of Food, 4 (1), 19 (2020).
- S. N. Dudd, R. P. Evershed, and A. M. Gibson, J. Archaeol. Sci., 26 (12), 1473 (1999).
- E. Casanova, T. D. Knowles, C. Ford, et al., Radiocarbon, 62 (6), 1679 (2020).
- Н. Н. Каширская, Л. Н. Плеханова, С. Н. Удальцов и др., Биофизика, 62 (6), 1235 (2017). doi: 10.1134/S0006350917060094
- Н. Н. Каширская, Л. Н. Плеханова, Е. В. Чернышева и др., Почвоведение, № 1, 89 (2020). doi: 10.1134/S0032180X20010098.
- M. W. I. Schmidt, M. S. Torn, S. Abiven, et al., Nature, 478, 49 (2011).
- В. М. Семенов, А. C. Тулина, Н. А. Семенова и Л. А. Иванникова, Почвоведение, № 4, 393 (2013). doi: 10.7868/S0032180X13040114
- M. Von Lutzow, I. Kogel-Knabner, B. Ludwig, et al., J. Plant Nutr. Soil Sci., 171, 111 (2008).
- В. А. Намиот, А. В. Батяновский, И. В. Филатов и др., Биофизика, 61 (1), 54 (2016).
- Е. В. Чернышева, М. А. Амирян, М. В. Ельцов и др., Ethnography, 18 (4), 993 (2022).
- Е. В. Чернышева, Н. Н. Каширская и К. С. Дущанова, Вестн. археологии, антропологии и этнографии, № 2 (61), 113 (2023).
- L. Casas-Godoy, S. Duquesne, F Bordes, et al., Methods Mol. Biol., 861, 3 (2012). doi: 10.1007/978-1-61779-600-5_1
- А. М. Безбородов и Н. А. Загустина, Прикладная биохимия и микробиология, 50 (4), 347 (2014).
- F. Akram, A. S. Mir, and A. Roohi, Mol. Biotechnol., 65, 521 (2023). doi: 10.1007/s12033-022-00592-z
- T. C. A. Hitch and T. Clavel, PeerJ., 7, e7249 (2019). doi: 10.7717/peerj.7249
- В. С. Гамаюрова, К. Л. Шнайдер и М. Е. Зиновьева, Вестн. Казанского технологич. ун-та, 17 (24), 175 (2014).
- A. Glogauer, V. P. Martini, H. Faoro, Microb. Cell Fact. 10, 54 (2011). doi: 10.1186/1475-2859-10-54
- P. Tirawongsaroj, R. Sriprang, P. Harnpicharnchai, et al., J. Biotechnol., 133 (1), 42 (2008). doi: 10.1016/j.jbiotec.2007.08.046
- M. Parapouli, A. Foukis, P. Y. Stergiou, et al., J. Biol. Res.-Thessaloniki, 25, 4 (2018). doi: 10.1186/s40709-018-0074-6
- Е. В. Чернышева, А. В. Борисов и В. Ю. Малашев, Краткие сообщения Института археологии, № 263, 105 (2021). doi: 10.25681/IARAS.0130-2620.263.105-116
- Н. Н. Каширская, Е. В. Чернышева и В. Ю. Малашев, История, археология и этнография Кавказа, 19 (1), 150 (2023).
- Т. Э. Хомутова, Т. С. Демкина, А. В. Борисов и Н. И. Шишлина, Почвоведение, № 2, 239 (2017). doi: 10.7868/S0032180X1702006X
- Л. Н. Плеханова, С. Н. Удальцов, А. С. Сыроватко и Н. Н. Каширская, Биофизика, 68 (5), 999 (2023). doi: 10.31857/S0006302923050204
- L. N. Plekhanova, in Proc. 19th Int. Multidisc. Sci. Geoconf. SGEM 2018, 18 (5.2), 173 (2018). doi: 10.5593/sgem2018/5.2/S20.023
- T. E. Khomutova and A. V. Borisov, J. Arid Environ., 171, 104004 (2019). doi: 10.1016/j.jaridenv.2019.104004
- В. А. Демкин, Т. С. Демкина и С. Н. Удальцов, Вестн. археологии, антропологии и этнографии, № 2 (25), 148 (2014).
- C.Ruiz, S. Falcocchio, E. Xoxi, et al., Biochim. Biophys. Acta - General Subjects, 1672 (3), 184 (2004).
- J. L. Smith and J. A. Alford, Appl. Microbiol., 14 (5), 699 (1966).
- P. Nobmann, A. Smith, J. Dunne, et al., Int. J. Food Microb., 128 (3), 440 (2009).
- C. Q. Sun, C. J. O'Connor, and A. M. Roberton, Chem.-biol.Interactions, 140 (2), 185 (2002).
- C. Zhong, N. Zhu, Y. Zhu, et al., Eur. J. Pharmaceut. Sci., 141, 105123 (2020).
- K. Sugimoto, H. Tanaka, R. Moriyama, Biocontrol Sci., 3 (1), 17 (1998).
- J. Lalman and D. M. Bagley, Water Res., 36 (13), 3307 (2002).
- C. S. Hwu, S. K. Tseng, C. Y. Yuan, et al., Water Res., 32 (5), 1571 (1998).
- J. Palatsi, M. Laureni, M. V. Andres, et al., Bioresource Technol., 100 (20), 4588 (2009).
- M. A. Pereira, O. C. Pires, M. Mota, and M. M. Alves, Biotechnology Bioengineer., 92 (1), 15 (2005).
- J. Palatsi, M. Vinas, M. Guivernau, Bioresource Technol., 102 (3), 2219 (2011).
- S. M. Iskander, Y. M. Amha, P. Wang, et al., Front. Bioengineer. Biotechnol., 9, 613626 (2021).
- Z. Guo, M. Usman, S. A. Alsareii, et al., Bioresource Technol., 337, 125383 (2021).
- H. Wang, Q. Yan, X. Zhong, et al., Bioresource Technol., 386, 129538 (2023).
- S. Amir, G. Merlina, E. Pinelli, et al., J. Hazardous Mater., 159 (2-3), 593 (2008).
- R. Gaur, R. Hemamalini, and S. Khare, in Current Developments in Biotechnology and Bioengineering, Ed. by A. N. S. Pandey and C. R. Soccol (Elsevier, 2017), p. 175.
- В. Ю. Малашев, Р. Г. Магомедов, Ф. С. Дзуцев и др., Археологические открытия, 2018, 342 (2020).
- Д. С. Коробов, В. Ю. Малашев, и Й. Фассбиндер, Краткие сообщения Института археологии, № 260, 441 (2020).
- A. Cowie, V. E. Lonergan, F. S. M. Rabbi, et al., Soil Res., 51, 707 (2013). doi: 10.1071/SR13043
- A. J. Margenot, Y. Nakayama, and S. J. Parikh, Soil Biol. Biochem., 125, 350 (2018). doi: 10.1016/j.soil-bio.2017.11.006
- S. Zhao, Y. Guo, Q. Sheng, and Y. Shyr, BioMed Res.Int., 2014, 986048 (2014). doi: 10.1155/2014/986048
- Д. А. Белинская и Н. В. Гончаров, Биоорган. химия, 46 (3), 247 (2020). DOI: 10.31857/ S0132342320030045