Alteration of enzyme-substrate interactions by ultra-high dilutions of the substrate

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

The effects of different ultra-high dilutions of luciferin ranged from 1010 to 10102 on luciferin-luciferase reaction of fireflies were investigated. Luciferin substrate concentration varied from 0.0025 nM to 2.5 nM and ATP concentration remained unchanged. This study evaluated the bioluminescence intensity after addition of ultra-high dilutions of luciferin or ultra-high dilutions of water to the bioluminescent system and some multidirectional and significant differences were found. The number of ultra-high dilutions from studied starting materials, for which significant differences were found, increased sharply with decreasing substrate concentration to 0.0025 nM. No differences were found when the effects of ultra-high dilutions of a non-specific control imidazole and ultra-high dilutions of water on the signal intensity were compared. Our results suggest that ultra-high dilutions of luciferin can specifically inhibit the luciferin-luciferase reaction.

Авторлар туралы

G. Lomakina

Lomonosov Moscow State University

Moscow, Russia

N. Ugarova

Lomonosov Moscow State University

Email: nugarova@gmail.com
Moscow, Russia

Әдебиет тізімі

  1. A. Ouertani, M. Neifar, R. Ouertani, et al., Adv. Tissue Eng. Regen Med. Open Access, 5, 85 (2019).
  2. Z. M. Kaskova, A. S. Tsarkova, and I. V. Yampolsky, Chem. Soc. Rev., 45 (21), 6048 (2016).
  3. L. J. Kricka, Anal. Biochem., 175 (1), 14 (1988).
  4. A. Pandey, P. Nigam, C. R. Soccol, et al., Biotechnol. Appl. Biochem., 31 (2), 135 (2000).
  5. N. N. Ugarova and G. Y. Lomakina, Moscow University Chem. Bull., 75, 15 (2020).
  6. D. V. Smirnova and N. N. Ugarova, Comb. Chem. High Throughput Screen., 18 (10), 946 (2015).
  7. P. M. de Souza and P. de Oliveira Magalhaes, Brazil. J. Microbiol., 41, 850 (2010).
  8. S. Raveendran, B. Parameswaran, S. B. Ummalyma, et al., Food Technol. Biotechnol., 56 (1), 16 (2018).
  9. V. I. Bruskov, A. V. Chernikov, et al., Phys. Wave Phen., 28 (2), 103 (2020).
  10. I. A. Scherbakov, I. V. Baimler, S. V. Gudkov, et al., Dokl. Physics, 65 (8), 9 (2020).
  11. S. V. Gudkov, G. A. Lyakhov, V. I. Pustovoy, et al., Phys. Wave Phen., 27 (2), 141 (2019).
  12. S. V. Gudkov, N. V. Penkov, I. V. Baimler, et al., Int. J. Mol. Sci., 21, 8033 (2020).
  13. I. V. Baymler, S. V. Gudkov, R. M. Sarimov, et al., Dokl. Physics, 65 (1), 5 (2020).
  14. W. Lauterborn, Appl. Phys. Lett., 21 (1), 27 (1972).
  15. N. F. Bunkin and F. V. Bunkin, Laser Physics, 3 (1), 63 (1993).
  16. A. Mai-Prochnow, R. Zhou, T. Zhang, et al., NPJ Biofilms and Microbiomes, 7, 11 (2021).
  17. Y. M. Zhao, A. Patange, D. W. Sun, et al., Comprehensive Reviews in Food Science and Food Safety, 19 (6), 3951 (2020).
  18. A. Scherbakov, Phys.Wave Phen., 29 (2), 89 (2021).
  19. I. S. Ryzhkina, L. I. Murtazina, Y. V. Kiseleva, et al., Dokl. Phys. Chem., 462 (1), 110 (2015).
  20. S.V. Gudkov, I.V. Baimler, O.V. Uvarov, et al., Front. Physics, 8, 622551 (2020).
  21. E. Don, O. Farafonova, S. Pokhil, et al., Sensors, 16, 10 (2016).
  22. E. S. Don, S. A. Bobrovnik, G. Sherriff, et al., J. Immunoassay Immunochem., 40 (3), 250 (2019).
  23. O. Epstein, Symmetry, 10 (4), 14 (2018).
  24. P. Bellavite, M. Marzotto, D. Olioso, et al., Homeopathy, 103 (1), 22 (2014).
  25. P. Muller, E. Ignatz, S. Kiontke, et al., Chem. Sci., 9 (5), 1200 (2017).
  26. S. A. Tarasov, E. A. Gorbunov, E. S. Don, et al., J. Immunol., 205 (5), 1345 (2020).
  27. P. S. Chikramane, D. Kalita, A. K. Suresh, et al., Langmuir, 28 (45), 15864 (2012).
  28. N. F. Bunkin, A. V. Shkirin, S. N. Chirikov, et al., ACS Omega, 5 (24), 14689 (2020).
  29. N. F. Bunkin, A. V. Shkirin, N. V. Penkov, et al., Phys. Wave Phen., 27 (2), 102 (2019).
  30. R. Bell, and G. E. Schwartz, Front.Biosci. (Schol Ed.), 5 (2), 685 (2013).
  31. S. V. Gudkov, G. A. Lyakhov, V. I. Pustovoy, et al., Phys. Wave Phen., 29 (2), 108 (2021).
  32. G. A. Lyakhov, and I. A. Scherbakov, Phys. Wave Phen., 27 (2), 79 (2019).
  33. V. Elia, G. Ausanio, F. Gentile, et al., Homeopathy, 103 (1), 44 (2014).
  34. E. Freier, S. Wolf, and K. Gerwert, Proc. Natl. Acad. Sci. USA, 108 (28), 11435 (2011).
  35. K. Haider, L. Wickstrom, S. Ramsey, et al., J. Phys. Chem. B, 120 (34), 8743 (2016).
  36. T. H. Plumridge and R. D. Waigh, J. Pharm. Rharmacol., 54 (9), 1155 (2002).
  37. T. M. Raschke, Curr. Opin. Struct. Biol., 16 (2), 152 (2006).
  38. R. A. Morton, T. A. Hopkins, and H. H. Seliger, Biochemistry, 8 (4), 1598 (1969).
  39. M. I. Koksharov and N. N. Ugarova, Photochem. Photobiol. Sci., 10 (6), 931 (2011).
  40. К. А. Сарбашев и Е. О. Химич, Свидетельство №2022665255. TenzometryUnit (программа для ЭВМ), 03.08.2022.
  41. V. V. Novikov and E. V. Yablokova, Field. Appl. Sci., 12, 5185 (2022).
  42. K. V. Wood, Available online: https://world-wide.promega.com/resources/pubhub/enotes/the-bioluminescence-advantage/(accessed on 23.12.2020).
  43. Food and Drug Administration, ORA Laboratory Manual Volume II. 2020, ORA-LAB.5.4.5.
  44. US Department of Health and Human Services, C.o.V.M. Food and Drug Administration. 2015, VICH GL49(R).
  45. M. Pschenitza, E. S. Gavrilova, C. Tarasov, et al., Int. Immunopharmacol., 21 (1), 225 (2014).
  46. N. N. Ugarova, J. Biolumin. Chemilumin., 4 (1), 406 (1989).
  47. W. D. McElroy, H. H. Seliger, and E. H. White, Photochem. Photobiol., 10 (3), 153 (1669).
  48. J. C. Cazin, M. Cazin, J. L. Gaborit, et al., Hum. Toxicol., 6 (4), 315 (1987).
  49. E. B. Burlakova, A. A. Konradova, and E. L. Maltseva, Chem. Physics, 22, 106 (2003).
  50. J. M. Saa and A. Frontera, ChemPhysChem., 21 (4), 313 (2020).
  51. I. V. Berezin, L. Y. Brovko, and N. N. Ugarova, Rus. J. Bioorg. Chem., 3 (12), 1589 (1977).
  52. S. D. Banik, M. Nordblad, J. M. Woodley, et al., Catalysts, 7 (8), 18 (2017).
  53. RCSB PDB, Available online: https://www.rcsb.org/structure/2D1R (accessed on 19.05.2020).
  54. V. V. Goncharuk, T. V. Pletneva, E. V. Uspenskaya, et al., J. Water Chem. Technol., 39 (6), 325 (2017).
  55. K. Johnson, arXiv: Atomic and Molecular Clusters (2009).

© Russian Academy of Sciences, 2023

Осы сайт cookie-файлдарды пайдаланады

Біздің сайтты пайдалануды жалғастыра отырып, сіз сайттың дұрыс жұмыс істеуін қамтамасыз ететін cookie файлдарын өңдеуге келісім бересіз.< / br>< / br>cookie файлдары туралы< / a>