Effects of Macromolecular Crowding in Erythrocyte Cytoplasm

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Abstract

Using Raman spectroscopy, fluorescence spectroscopy, and time-correlated single-photon counting, it was shown that increasing the incubation temperature of human erythrocytes enhances the homogeneity of hemoglobin distribution in the cytoplasm and reduces globin molecule packing density. This likely results from the cellular “macromolecular crowding” effect. Observed conformational changes are specific to intracellular Hb and independent of the plasma membrane surface potential. Multiple pools of hemoglobin with varying structure and dynamics are proposed, they form clusters, creating heterogeneous hemoglobin distribution. The role of these effects in oxygen transport and regulatory signaling is discussed.

About the authors

O. V Slatinskaya

Department of Biology, Lomonosov Moscow State University

Moscow, Russia

E. Yu Parshina

Department of Biology, Lomonosov Moscow State University

Moscow, Russia

A. I Yusipovich

Department of Biology, Lomonosov Moscow State University

Moscow, Russia

N. A Brazhe

Department of Biology, Lomonosov Moscow State University

Moscow, Russia

A. B Rubin

Department of Biology, Lomonosov Moscow State University

Moscow, Russia

G. V Maksimov

Department of Biology, Lomonosov Moscow State University; University of Science and Technology MISIS

Email: gmaksimov@mail.ru
Moscow, Russia; Moscow, Russia

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