Study of the Distribution of Subcellular Structures in Algae Cells Using Optical Laser Tomography

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Abstract

Using the developed method of optical laser tomography, the possibility of recording and analyzing the threedimensional distribution of subcellular organelles in eukaryotic algae (C. reinhardtii) was proved. The method allows analyzing the redistribution of subcellular organelles (nucleus, chloroplast) in consecutive slices of one cell in norm and under the influence of modifiers of its functional state. According to the authors, the proposed approach will allow not only to study the dynamics of distribution of intracellular structures (nucleus, thylakoids, mitochondria, etc.), but also changes of a single structure during cell functioning.

About the authors

A. A Samoilenko

All-Russian Research Institute for Optical-Physical Measurements

Moscow, Russia

G. G Levin

All-Russian Research Institute for Optical-Physical Measurements

Moscow, Russia

A. A Volgusheva

Lomonosov Moscow State University

Moscow, Russia

A. P Kazakov

Lomonosov Moscow State University

Moscow, Russia

G. V Maksimov

Lomonosov Moscow State University; National University of Science and Technology “MISIS”

Email: gmaksimov@mail.ru
Moscow, Russia; Moscow, Russia

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