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Thermal Processes Associated with Electrophoresis under Conditions of High Ionic Strength
- Authors: Sirota N.P1, Khizhnyak E.P1, Khizhnyak L.N1, Sirota T.V1, Kuznetsova E.A1
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Affiliations:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- Issue: Vol 70, No 1 (2025)
- Pages: 137-143
- Section: Complex systems biophysics
- URL: https://journals.rcsi.science/0006-3029/article/view/285392
- DOI: https://doi.org/10.31857/S0006302925010162
- EDN: https://elibrary.ru/LUMFXB
- ID: 285392
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Abstract
Temperature heterogeneity of the electrophoretic solution, dynamically changing during electrophoresis in the gel platform area, was demonstrated by us earlier. In order to understand the reasons for this effect, we used U-shaped inserts in the wells of the electrophoretic chamber, protruding above the surface of the electrophoretic solution, but not affecting the current flow. The conducted study showed that the migration of heated masses of solution is caused by different rates of hydrogen and oxygen production on platinum electrodes. Using technique of real-time thermal imaging, it was demonstrated that in the presence of U-shaped inserts, heating was uniform up to 7°С throughout the entire volume of the solution located above the gel platform. Heating of the liquid in the well area occurred with less dynamics and was not identical. The heating of the liquid in the cathode well occurs insignificantly (only 1.5°C), and at the anode the increase is 2.5°С. We believe that temperature homogeneity will be of significant importance for reducing the variability of the results obtained by the Comet assay (DNA comet) method on preparations subjected to alkaline electrophoresis.
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About the authors
N. P Sirota
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: sirota@iteb.ru
Pushchino, Moscow
E. P Khizhnyak
Institute of Theoretical and Experimental Biophysics, Russian Academy of SciencesPushchino, Moscow
L. N Khizhnyak
Institute of Theoretical and Experimental Biophysics, Russian Academy of SciencesPushchino, Moscow
T. V Sirota
Institute of Theoretical and Experimental Biophysics, Russian Academy of SciencesPushchino, Moscow
E. A Kuznetsova
Institute of Theoretical and Experimental Biophysics, Russian Academy of SciencesPushchino, Moscow
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