电邮这篇文章 Formation of Heterotetrameric Potassium Channels Kv1.1–Kv1.2 in Neuro-2a Cells: Analysis by the Förster Resonance Energy Transfer Technique
- 作者: Efremenko A.V1, Nekrasova O.V1, Feofanov A.V1,2
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隶属关系:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Lomonosov Moscow State University
- 期: 卷 70, 编号 1 (2025)
- 页面: 78-85
- 栏目: Cell biophysics
- URL: https://journals.rcsi.science/0006-3029/article/view/285385
- DOI: https://doi.org/10.31857/S0006302925010098
- EDN: https://elibrary.ru/LWZIFK
- ID: 285385
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详细
The subunits of the voltage-gated potassium channels Kv1.1 and Kv1.2 can form both homo- and heterotetrameric channels in cells. This significantly affects the functional properties and localization of the formed Kv1 channels. Confocal microscopy based on Förster resonance energy transfer was used to study the formation of Kv1 channels during co-expression of subunits Kv1.1(S369T) and Kv1.2(S371T), fused with the fluorescent protein mKate2 and TagCFP, respectively, in murine neuroblastoma Neuro-2a cells. Due to mutation, these subunits provide enhanced transfer of Kv1 channels in plasma membrane. It was found that TagCFP-Kv1.1(S369T) and mKate2-Kv1.2(S371T) effectively form heterochannels that are localized both on the membrane and in the cytoplasm of cells. In the absence of the S369T mutation, heterochannels are not embedded in the membrane, which indicates the need for auxiliary factors for the transfer of native heterochannels into the cell membrane. In addition to heterochannels, homotetrameric channels are also formed in cells, but the effectiveness of the formation of heterochannels is much higher.
作者简介
A. Efremenko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscow, Russia
O. Nekrasova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscow, Russia
A. Feofanov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Lomonosov Moscow State University
Email: avfeofanov@yandex.ru
Moscow, Russia; Moscow, Russia
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