Stoichiometry of the Formation of Physiologically Active Cytochrome C Complexes with Cardiolipin

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Abstract

The major goal of this work was to study the stoichiometry of the interaction of cytochrome C with tetraoleoyl- or tetramyristoyl-cardiolipin. Complexes of cytochrome C with phospholipid play one of the main roles in apoptotic processes. This study was conducted using fluorescently labeled phosphatidylcholine (BODIPY-PC). The fluorescence quenching of the BODIPY-PC probe after its interaction with cytochrome C was measured. Calculations of the fluorescence quenching results allowed us to determine the stoichiometry of the cytochrome C complexes formed with phospholipids. For complexes containing tetraoleoyl, stoichiometry corresponded to the ratio tetraoleoyl : cytochrome C equal to 25 ± 7, and for tetramyristoyl the ratio tetramyristoyl : cytochrome C – 7 ± 2. Obtained results may indicate a difference in the affinity of cytochrome C for saturated and unsaturated types of cardiolipin, despite the same electrostatic characteristics of the molecules. It can be expected that the discovered features of the interaction of cytochrome C with cardiolipin will eventually help determine the intensity and direction of apoptotic reactions.

About the authors

G. O Stepanov

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Email: stepanov_go@rsmu.ru
Moscow, Russia

G. K Vladimirov

Lomonosov Moscow State University; Kurchatov Complex of Crystallography and Photonics, National Research Centre "Kurchatov Institute"

Moscow, Russia; Moscow, Russia

I. V Kirilina

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Moscow, Russia

A. R Guselnikova

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Moscow, Russia

V. I Tihonova

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Moscow, Russia

P. D Vinogradova

Lomonosov Moscow State University

Moscow, Russia

A. N Osipov

N.I. Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation

Moscow, Russia

Yu. A Vladimirov

Lomonosov Moscow State University; Kurchatov Complex of Crystallography and Photonics, National Research Centre "Kurchatov Institute"

Moscow, Russia; Moscow, Russia

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