Photodynamic and Plasmonic Photothermal Combination Therapy in a Rat Model of Transplanted Tumors

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Abstract

The aim of this study is to develop a combined technology which integrates plasmonic photothermal therapy and photodynamic therapy of transplanted cholangiocarcinoma РС-1 in rats. For photodynamic therapy, rats received intratumoral injection of 2 mg/kg indocyanine green diluted in a 1:100 volume ratio with polyethylene glycol. For plasmonic photothermal therapy, the intratumoral injectate volume of gold nanorods (400 μg/ml) coated with polyethylene glycol for tumor was drawn up to be 30% to the tumor volume. One hour after injections, the tumor was irradiated percutaneously with an 808 nm infrared diode laser at a power density of 2.3 W/cm2 for 15 min. Animals were excluded from the experiment 72 and 21 days after therapy. Morphological studies of the tumor were performed on sections stained by standard protocols and immunohistochemical methods. A significant rise in tumor temperature, up to 60 ± 4.1°C, was noted during plasmonic photothermal therapy combined with photodynamic therapy. In 72 hours, the pronounced necrotic changes in the tumor tissue were observed, the residual tumor cell foci were found only in the periphery of the tumor. A significant inhibition of tumor growth was observed 21 days after therapy; the tumor growth inhibition index by tumor mass was 77.4%.

About the authors

A. B Bucharskaya

V.I. Razumovsky Saratov State Medical University, Ministry of Health of the Russian Federation; National Research Saratov State University named after N.G. Chernyshevsky; National Research Tomsk State University

Email: allaalla_72@mail.ru
Saratov, Russia; Saratov, Russia; Tomsk, Russia

N. A Navolokin

V.I. Razumovsky Saratov State Medical University, Ministry of Health of the Russian Federation; National Research Saratov State University named after N.G. Chernyshevsky

Saratov, Russia; Saratov, Russia

D. A Mudrak

V.I. Razumovsky Saratov State Medical University, Ministry of Health of the Russian Federation

Saratov, Russia

G. N Maslyakova

V.I. Razumovsky Saratov State Medical University, Ministry of Health of the Russian Federation; National Research Saratov State University named after N.G. Chernyshevsky

Saratov, Russia; Saratov, Russia

B. N Khlebtsov

Institute of Biochemistry and Physiology of Plants and Microorganisms

Saratov, Russia

N. G Khlebtsov

National Research Saratov State University named after N.G. Chernyshevsky; Institute of Biochemistry and Physiology of Plants and Microorganisms

Saratov, Russia; Saratov, Russia

V. D Genin

National Research Saratov State University named after N.G. Chernyshevsky; National Research Tomsk State University

Saratov, Russia; Tomsk, Russia

E. A Genina

National Research Saratov State University named after N.G. Chernyshevsky;

Saratov, Russia; Tomsk, Russia

V. V Tuchin

National Research Saratov State University named after N.G. Chernyshevsky; National Research Tomsk State University

Saratov, Russia; Tomsk, Russia

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