Email this article Superoxide Generating Activity of Nicotinamide Coenzymes
- Authors: Sirota T.V1
-
Affiliations:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- Issue: Vol 69, No 1 (2024)
- Pages: 24-31
- Section: Molecular biophysics
- URL: https://journals.rcsi.science/0006-3029/article/view/257121
- DOI: https://doi.org/10.31857/S0006302924010032
- EDN: https://elibrary.ru/RIBSKI
- ID: 257121
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Abstract
It has been shown that nicotinamide coenzymes (NADPH, NADH, NADP+, NAD+) are capable of generating superoxide anions (O2─●) in an alkaline environment. The superoxide-generating activity of coenzymes is associated with high pH values and is sensitive to SOD. However, nicotinamide itself, being a functional part of coenzyme molecules, does not have this property. Polarographic studies have shown that in the presence of coenzymes, molecular oxygen is consumed from the buffer, namely oxygen activation occurs due to the formation of O2-●. Based on the results obtained and in accordance with the literature, our observations suggest that the formation of adducts of nicotinamide, which is part of the coenzyme molecule, and hydroxyl anions (OH−) may lead to the formation of O2─●. Under mild conditions in the organism, the studied coenzymes, while performing their main functions, are expected to generate superoxide, meaning that they can be signaling molecules.
About the authors
T. V Sirota
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: sirotatv@rambler.ru
Institutskaya ul. 3, Pushchino, Moscow Region, 142290, Russia
References
- Сирота Т. В. Генерация супероксида никотинамидными коферментами. Biomed. Chem.: Research and Methods, 6 (1), e00188 (2023). doi: 10.18097/BMCRM00188
- Сирота Т. В. и Сирота Н. П. К механизму активации кислорода в химических и биологических системах. Биофизика, 67 (1), 5–13 (2022). doi: 10.31857/S000630292201001X
- Hayyan M., Hashim M. A., and Al Nashef I. M. Superoxide ion: generation and chemical implications. Chem.Rev., 116 (5), 3029–3085 (2016). doi: 10.1021/acs.chemrev.5b00407
- Andrés C. M. C., Pérez de la Lastra J. M., Andrés Juan C., Plou F. J., and Pérez-Lebeña E. Superoxide anion chemistry – its role at the core of the innate immunity. Int. J. Mol. Sci., 24 (3), 1841, (2023). doi: 10.3390/ijms24031841
- Ray P. D., Huang Bo-W., and Tsuji Y. Reactiv oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal., 24 (5), 981–990 (2012). doi: 10.1016/j.cellsig.2012.01.008
- Weihai Y. NAD+ and NADH in cellular functions and cell death, Front. Biosci., 11, 3129–3148 (2006). doi: 10.2741/2038
- Pollak N., Dölle C., and Ziegler M. The power to reduce: pyridine nucleotides-small molecules with a multitude of functions. Biochem. J., 402 (2), 205–218 (2007). doi: 10.1042/BJ20061638
- Мецлер Д. Биохимия: химические реакции в живой клетке (Мир, М., 1980), т. 2, сс. 250–253.
- http://vmede.org/sait/?page=15&id=Bioorganicheskaja_himija_tykavkina_2010&menu=Bioorganicheskaja_himija_tykavkina_2010
- Sirota T. V. A novel approach to study the reaction of adrenaline autooxidation: a possibility for polarographic determination of superoxide dismutase activity and antioxidant properties of various preparations. Biochemistry (Moscow). Suppl. Ser. B: Biomedical Chemistry, 5 (3), 253–259 (2011). doi: 10.1134/S1990750811030139
- Сирота Т. В. Использование нитросинего тетразолия в реакции автоокисления адреналина для определения активности супероксидисмутазы. Биомедицинская химия, 59 (4), 399–410 (2013). doi: 10.18097/pbmc20135904399
- Сирота Т. В. Цепная реакция автоокисления адреналина – модель хиноидного окисления катехоламинов. Биофизика, 65 (4), 646–655 (2020). doi: 10.31857/S0006302920040031
- Misra H. P. and Fridovich I. J. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem., 247 (10), 3170–3175 (1972).
- Altman F. P. Tetrazolium saits and formazans. Progr. Histochem. Cytochem., 9 (3), 1–56 (1976). doi: 10.1016/S0079-6336(76)80015-0
- Guilbertt C. C. and Johnson S. L. Isolation and characterization of the fluorescent alkali product from diphosphopyridine nucleotide. Biochemistry, 10 (12), 2313–2316 (1971). doi: 10.1021/bi00788a021
- Valko M., Leibfritz D., Moncol J., Cronin M.T.D., Mazur M., and Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 39 (1), 44–84 (2007). doi: 10.1016/j.biocel.2006.07.001
