Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional protein domains: Synthesis in an Escherichia coli heterologous expression system
- 作者: Karyagina A.1,2,3, Boksha I.1,4, Grunina T.1, Demidenko A.1, Poponova M.1, Sergienko O.1,3, Lyashchuk A.1, Galushkina Z.1, Soboleva L.1, Osidak E.5, Bartov M.1, Gromov A.1, Lunin V.1,3
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隶属关系:
- Gamaleya Center of Epidemiology and Microbiology
- Belozersky Institute of Physico-Chemical Biology
- Russian Research Institute of Agricultural Biotechnology
- Mental Health Research Center
- IMTEK Ltd.
- 期: 卷 82, 编号 5 (2017)
- 页面: 613-624
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151384
- DOI: https://doi.org/10.1134/S0006297917050091
- ID: 151384
如何引用文章
详细
Two variants of recombinant human bone morphogenetic protein-2 (rhBMP-2) with additional N-terminal protein domains were obtained by expression in E. coli. The N-terminal domains were s-tag (15-a.a. oligopeptide from bovine pancreatic ribonuclease A) and lz (leucine zipper dimerization domain from yeast transcription factor GCN4). The s-tag-BMP-2 and lz-BMP-2 were purified by a procedure that excluded a long refolding stage. The resulting dimeric proteins displayed higher solubility compared to rhBMP-2 without additional protein domains. Biological activity of both proteins was demonstrated in vitro by induction of alkaline phosphatase in C2C12 cells, and the activity of s-tag-BMP-2 in vivo was shown in various experimental animal models.
作者简介
A. Karyagina
Gamaleya Center of Epidemiology and Microbiology; Belozersky Institute of Physico-Chemical Biology; Russian Research Institute of Agricultural Biotechnology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098; Moscow, 119992; Moscow, 127550
I. Boksha
Gamaleya Center of Epidemiology and Microbiology; Mental Health Research Center
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098; Moscow, 115522
T. Grunina
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
A. Demidenko
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
M. Poponova
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
O. Sergienko
Gamaleya Center of Epidemiology and Microbiology; Russian Research Institute of Agricultural Biotechnology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098; Moscow, 127550
A. Lyashchuk
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
Z. Galushkina
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
L. Soboleva
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
E. Osidak
IMTEK Ltd.
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 121552
M. Bartov
Gamaleya Center of Epidemiology and Microbiology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
A. Gromov
Gamaleya Center of Epidemiology and Microbiology
编辑信件的主要联系方式.
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098
V. Lunin
Gamaleya Center of Epidemiology and Microbiology; Russian Research Institute of Agricultural Biotechnology
Email: alexander.v.gromov@gmail.com
俄罗斯联邦, Moscow, 123098; Moscow, 127550
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