Subcellular localization and detection of Tobacco mosaic virus ORF6 protein by immunoelectron microscopy
- 作者: Erokhina T.1, Lazareva E.2, Richert-Pöggeler K.3, Sheval E.2, Solovyev A.2, Morozov S.2
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Belozersky Institute of Physico-Chemical Biology
- Julius-Kühn-Institut
- 期: 卷 82, 编号 1 (2017)
- 页面: 60-66
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151221
- DOI: https://doi.org/10.1134/S0006297917010060
- ID: 151221
如何引用文章
详细
Members of the genus Tobamovirus represent one of the best-characterized groups of plant positive, single stranded RNA viruses. Previous studies have shown that genomes of some tobamoviruses contain not only genes coding for coat protein, movement protein, and the cistron coding for different domains of RNA-polymerase, but also a gene, named ORF6, coding for a poorly conserved small protein. The amino acid sequences of ORF6 proteins encoded by different tobamoviruses are highly divergent. The potential role of ORF6 proteins in replication of tobamoviruses still needs to be elucidated. In this study, using biochemical and immunological methods, we have shown that ORF6 peptide is accumulated after infection in case of two isolates of Tobacco mosaic virus strain U1 (TMV-U1 common and TMV-U1 isolate A15). Unlike virus particles accumulating in the cytoplasm, the product of the ORF6 gene is found mainly in nuclei, which correlates with previously published data about transient expression of ORF6 isolated from TMV-U1. Moreover, we present new data showing the presence of ORF6 genes in genomes of several tobamoviruses. For example, in the genomes of other members of the tobamovirus subgroup 1, including Rehmannia mosaic virus, Paprika mild mottle virus, Tobacco mild green mosaic virus, Tomato mosaic virus, Tomato mottle mosaic virus, and Nigerian tobacco latent virus, sequence comparisons revealed the existence of a similar open reading frame like ORF6 of TMV.
作者简介
T. Erokhina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: tne@mx.ibch.ru
俄罗斯联邦, Moscow, 117997
E. Lazareva
Belozersky Institute of Physico-Chemical Biology
Email: tne@mx.ibch.ru
俄罗斯联邦, Moscow, 119991
K. Richert-Pöggeler
Julius-Kühn-Institut
Email: tne@mx.ibch.ru
德国, Messeweg 11/12, Braunschweig, 38104
E. Sheval
Belozersky Institute of Physico-Chemical Biology
Email: tne@mx.ibch.ru
俄罗斯联邦, Moscow, 119991
A. Solovyev
Belozersky Institute of Physico-Chemical Biology
Email: tne@mx.ibch.ru
俄罗斯联邦, Moscow, 119991
S. Morozov
Belozersky Institute of Physico-Chemical Biology
Email: tne@mx.ibch.ru
俄罗斯联邦, Moscow, 119991
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