Immuno-PCR: achievements and perspectives
- 作者: Ryazantsev D.1, Voronina D.1, Zavriev S.1
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- 期: 卷 81, 编号 13 (2016)
- 页面: 1754-1770
- 栏目: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151199
- DOI: https://doi.org/10.1134/S0006297916130113
- ID: 151199
如何引用文章
详细
The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of “visualization” of antigen–antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.
作者简介
D. Ryazantsev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: d.yu.ryazantsev@gmail.com
俄罗斯联邦, Moscow, 117997
D. Voronina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: d.yu.ryazantsev@gmail.com
俄罗斯联邦, Moscow, 117997
S. Zavriev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: d.yu.ryazantsev@gmail.com
俄罗斯联邦, Moscow, 117997