电邮这篇文章 Staphylococcus simulans recombinant lysostaphin: Production, purification, and determination of antistaphylococcal activity
- 作者: Boksha I.1, Lavrova N.1, Grishin A.1, Demidenko A.1, Lyashchuk A.1, Galushkina Z.1, Ovchinnikov R.1, Umyarov A.1, Avetisian L.1, Chernukha M.1, Shaginian I.1, Lunin V.1, Karyagina A.1
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隶属关系:
- N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
- 期: 卷 81, 编号 5 (2016)
- 页面: 502-510
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150878
- DOI: https://doi.org/10.1134/S0006297916050072
- ID: 150878
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详细
Staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. A synthetic gene encoding S. simulans lysostaphin was cloned in Escherichia coli cells, and producer strains were designed. The level of produced biologically active lysostaphin comprised 6-30% of total E. coli cell protein (depending on E. coli M15 or BL21 producer) under batch cultivation conditions. New methods were developed for purification of lysostaphin without affinity domains and for testing its enzymatic activity. As judged by PAGE, the purified recombinant lysostaphin is of >97% purity. The produced lysostaphin lysed cells of Staphylococcus aureus and Staphylococcus haemolyticus clinical isolates. In vitro activity and general biochemical properties of purified recombinant lysostaphin produced by M15 or BL21 E. coli strains were identical to those of recombinant lysostaphin supplied by SigmaAldrich (USA) and used as reference in other known studies. The prepared recombinant lysostaphin represents a potential product for development of enzymatic preparation for medicine and veterinary due to the simple purification scheme enabling production of the enzyme of high purity and antistaphylococcal activity.
作者简介
I. Boksha
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
编辑信件的主要联系方式.
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
N. Lavrova
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
A. Grishin
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
A. Demidenko
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
A. Lyashchuk
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
Z. Galushkina
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
R. Ovchinnikov
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
A. Umyarov
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
L. Avetisian
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
M. Chernukha
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
I. Shaginian
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
V. Lunin
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
A. Karyagina
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
俄罗斯联邦, Moscow, 123098
