Мақаланы E-mail арқылы жіберу Staphylococcus simulans recombinant lysostaphin: Production, purification, and determination of antistaphylococcal activity
- Авторлар: Boksha I.1, Lavrova N.1, Grishin A.1, Demidenko A.1, Lyashchuk A.1, Galushkina Z.1, Ovchinnikov R.1, Umyarov A.1, Avetisian L.1, Chernukha M.1, Shaginian I.1, Lunin V.1, Karyagina A.1
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Мекемелер:
- N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
- Шығарылым: Том 81, № 5 (2016)
- Беттер: 502-510
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150878
- DOI: https://doi.org/10.1134/S0006297916050072
- ID: 150878
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Staphylococcus simulans lysostaphin is an endopeptidase lysing staphylococcus cell walls by cleaving pentaglycine cross-bridges in their peptidoglycan. A synthetic gene encoding S. simulans lysostaphin was cloned in Escherichia coli cells, and producer strains were designed. The level of produced biologically active lysostaphin comprised 6-30% of total E. coli cell protein (depending on E. coli M15 or BL21 producer) under batch cultivation conditions. New methods were developed for purification of lysostaphin without affinity domains and for testing its enzymatic activity. As judged by PAGE, the purified recombinant lysostaphin is of >97% purity. The produced lysostaphin lysed cells of Staphylococcus aureus and Staphylococcus haemolyticus clinical isolates. In vitro activity and general biochemical properties of purified recombinant lysostaphin produced by M15 or BL21 E. coli strains were identical to those of recombinant lysostaphin supplied by SigmaAldrich (USA) and used as reference in other known studies. The prepared recombinant lysostaphin represents a potential product for development of enzymatic preparation for medicine and veterinary due to the simple purification scheme enabling production of the enzyme of high purity and antistaphylococcal activity.
Авторлар туралы
I. Boksha
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Хат алмасуға жауапты Автор.
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
N. Lavrova
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
A. Grishin
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
A. Demidenko
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
A. Lyashchuk
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
Z. Galushkina
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
R. Ovchinnikov
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
A. Umyarov
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
L. Avetisian
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
M. Chernukha
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
I. Shaginian
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
V. Lunin
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
A. Karyagina
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology
Email: boksha_irina@mail.ru
Ресей, Moscow, 123098
