Attenuation of focal adhesion kinase reduces lipopolysaccharide-induced inflammation injury through inactivation of the Wnt and NF-κB pathways in A549 cells
- Авторы: Bai D.1, Cong S.1, Zhu L.1
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Учреждения:
- Jining No. 1 People’s Hospital
- Выпуск: Том 82, № 4 (2017)
- Страницы: 446-453
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151333
- DOI: https://doi.org/10.1134/S0006297917040058
- ID: 151333
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Аннотация
Overall analysis and understanding of mechanisms are of great importance for treatment of infantile pneumonia due to its high morbidity and mortality worldwide. In this study, we preliminarily explored the function and mechanism of focal adhesion kinase (FAK) in regulation of inflammatory response induced by lipopolysaccharides in A549 cells. Flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, quantitative reverse transcription polymerase chain reaction, and Western blot analysis were used to explore the correlation of FAK expression with cell apoptosis, viability, and the inflammatory cytokine activity in A549 cells. The results showed that knockdown of FAK enhanced cell viability, suppressed apoptosis, and decreased inflammatory cytokine activity. In addition, downregulation of FAK could activate the Wnt and nuclear factor κB signaling pathways. These findings suggest that FAK might be involved in progression of infantile pneumonia and could be a new therapeutic target for this disease.
Об авторах
D. Bai
Jining No. 1 People’s Hospital
Email: zhuliping5280@126.com
КНР, Jining, 272011
S. Cong
Jining No. 1 People’s Hospital
Email: zhuliping5280@126.com
КНР, Jining, 272011
L. Zhu
Jining No. 1 People’s Hospital
Автор, ответственный за переписку.
Email: zhuliping5280@126.com
КНР, Jining, 272011
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