Determination of size of folding nuclei of fibrils formed from recombinant Aβ(1-40) peptide
- Autores: Grigorashvili E.1, Selivanova O.1, Dovidchenko N.1, Dzhus U.1, Mikhailina A.1, Suvorina M.1, Marchenkov V.1, Surin A.1,2, Galzitskaya O.1
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Afiliações:
- Institute of Protein Research
- State Research Center for Applied Microbiology and Biotechnology
- Edição: Volume 81, Nº 5 (2016)
- Páginas: 538-547
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150891
- DOI: https://doi.org/10.1134/S0006297916050114
- ID: 150891
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Resumo
We have developed a highly efficient method for purification of the recombinant product Aβ(1-40) peptide. The concentration dependence of amyloid formation by recombinant Aβ(1-40) peptide was studied using fluorescence spectroscopy and electron microscopy. We found that the process of amyloid formation is preceded by lag time, which indicates that the process is nucleation-dependent. Further exponential growth of amyloid fibrils is followed by branching scenarios. Based on the experimental data on the concentration dependence, the sizes of the folding nuclei of fibrils were calculated. It turned out that the size of the primary nucleus is one “monomer” and the size of the secondary nucleus is zero. This means that the nucleus for new aggregates can be a surface of the fibrils themselves. Using electron microscopy, we have demonstrated that fibrils of these peptides are formed by the association of rounded ring structures.
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Sobre autores
E. Grigorashvili
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
O. Selivanova
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
N. Dovidchenko
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
U. Dzhus
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
A. Mikhailina
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
M. Suvorina
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
V. Marchenkov
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
A. Surin
Institute of Protein Research; State Research Center for Applied Microbiology and Biotechnology
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290; Obolensk, Moscow Region, 142279
O. Galzitskaya
Institute of Protein Research
Autor responsável pela correspondência
Email: ogalzit@vega.protres.ru
Rússia, Pushchino, Moscow Region, 142290
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