Isolation of homogeneous polysaccharide monooxygenases from fungal sources and investigation of their synergism with cellulases when acting on cellulose
- Autores: Bulakhov A.1, Gusakov A.1,2, Chekushina A.1, Satrutdinov A.1, Koshelev A.3, Matys V.3, Sinitsyn A.1,2
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Afiliações:
- Federal Research Center Fundamentals of Biotechnology
- Lomonosov Moscow State University
- Skryabin Institute of Biochemistry and Physiology of Microorganisms
- Edição: Volume 81, Nº 5 (2016)
- Páginas: 530-537
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150887
- DOI: https://doi.org/10.1134/S0006297916050102
- ID: 150887
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Resumo
Lytic polysaccharide monooxygenases (PMO) discovered several years ago are enzymes classified as oxidoreductases. In nature, they participate in microbial degradation of cellulose together with cellulases that belong to the hydrolytic type of enzymes (class of hydrolases). Three PMO from ascomycetes–Thielavia terrestris, Trichoderma reesei, and Myceliophthora thermophila–were isolated and purified to homogeneous state using various types of chromatography. The first two enzymes are recombinant proteins heterologously expressed by the Penicillium verruculosum fungus, while the third is a native PMO secreted by M. thermophila. When acting on microcrystalline cellulose, all these PMOs displayed synergism with the cellulase complex of the P. verruculosum fungus. Replacing 10% of cellulases (by protein concentration) with PMO in the presence of 6.25 mM gallic acid or 2.5 μM of cellobiose dehydrogenase from M. thermophila, used as electron donors for PMO, resulted in the 17-31% increase in the yield of reducing sugars after 24-48 h of the enzymatic reaction.
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Sobre autores
A. Bulakhov
Federal Research Center Fundamentals of Biotechnology
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Moscow, 119071
A. Gusakov
Federal Research Center Fundamentals of Biotechnology; Lomonosov Moscow State University
Autor responsável pela correspondência
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Moscow, 119071; Moscow, 119991
A. Chekushina
Federal Research Center Fundamentals of Biotechnology
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Moscow, 119071
A. Satrutdinov
Federal Research Center Fundamentals of Biotechnology
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Moscow, 119071
A. Koshelev
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Pushchino, Moscow Region, 142292
V. Matys
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Pushchino, Moscow Region, 142292
A. Sinitsyn
Federal Research Center Fundamentals of Biotechnology; Lomonosov Moscow State University
Email: avgusakov@enzyme.chem.msu.ru
Rússia, Moscow, 119071; Moscow, 119991
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