A glutamine/asparagine-rich fragment of Gln3, but not the full-length protein, aggregates in Saccharomyces cerevisiae
- Autores: Antonets K.1,2, Sargsyan H.1, Nizhnikov A.1,2,3
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Afiliações:
- Department of Genetics and Biotechnology
- Vavilov Institute of General Genetics, St. Petersburg Branch
- All-Russia Research Institute for Agricultural Microbiology
- Edição: Volume 81, Nº 4 (2016)
- Páginas: 407-413
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150843
- DOI: https://doi.org/10.1134/S0006297916040118
- ID: 150843
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Resumo
The amino acid sequence of protein Gln3 in yeast Saccharomyces cerevisiae has a region enriched with Gln (Q) and Asn (N) residues. In this study, we analyzed the effects of overexpression of Gln3 and its Q/N-rich fragment fused with yellow fluorescent protein (YFP). Being overexpressed, full-length Gln3-YFP does not form aggregates, inhibits vegetative growth, and demonstrates nuclear localization, while the Q/N-rich fragment (Gln3QN) fused with YFP forms aggregates that do not colocalize with the nucleus and do not affect growth of the cells. Although detergent-resistant aggregates of Gln3QN are formed in the absence of yeast prions, the aggregation of Gln3QN significantly increases in the presence of [PIN+] prion, while in the presence of two prions, [PSI+] and [PIN+], the percentage of cells with Gln3QN aggregates is significantly lower than in the strain bearing only [PIN+]. Data on colocalization demonstrate that this effect is mediated by interaction between Gln3QN aggregates and [PSI+] and [PIN+] prions.
Sobre autores
K. Antonets
Department of Genetics and Biotechnology; Vavilov Institute of General Genetics, St. Petersburg Branch
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034
H. Sargsyan
Department of Genetics and Biotechnology
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034
A. Nizhnikov
Department of Genetics and Biotechnology; Vavilov Institute of General Genetics, St. Petersburg Branch; All-Russia Research Institute for Agricultural Microbiology
Autor responsável pela correspondência
Email: ant.nizhnikov@gmail.com
Rússia, St. Petersburg, 199034; St. Petersburg, 199034; St. Petersburg, 196608 Pushkin
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