Lymphocyte Phosphatase-Associated Phosphoprotein Is a Substrate of Protein Kinase CK2
- Авторлар: Tsoy T.1,2, Kruglova N.1,2, Filatov A.1,2
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Мекемелер:
- Institute of Immunology National Research Center
- Faculty of Biology
- Шығарылым: Том 83, № 11 (2018)
- Беттер: 1380-1387
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151758
- DOI: https://doi.org/10.1134/S0006297918110081
- ID: 151758
Дәйексөз келтіру
Аннотация
Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a molecular partner of CD45 phosphatase that plays a key role in the regulation of antigen-specific activation of lymphocytes. The functions of LPAP still remain unknown. We believe that studying LPAP phosphorylation pathways could shed light on its functions. In this work, we studied the phosphorylation of LPAP ectopically expressed in non-lymphoid cells in order to determine the effect of LPAP interaction partners on its phosphorylation. We found that phosphorylation at Ser153 and Ser163 in non-hematopoietic HEK293 cells was conserved, while phosphorylation at Ser99 and Ser172 was almost absent. The pattern of LPAP phosphorylation in K562 erythroid and U937 myeloid cells expressing endogenous CD45 protein was similar to that observed in T and B lymphocytes. We demonstrated for the first time that LPAP is a substrate for protein kinase CK2 that phosphorylates it at Ser153, presumably ensuring LPAP resistance to degradation.
Негізгі сөздер
Авторлар туралы
T. Tsoy
Institute of Immunology National Research Center; Faculty of Biology
Email: avfilat@yandex.ru
Ресей, Moscow, 115522; Moscow, 119234
N. Kruglova
Institute of Immunology National Research Center; Faculty of Biology
Email: avfilat@yandex.ru
Ресей, Moscow, 115522; Moscow, 119234
A. Filatov
Institute of Immunology National Research Center; Faculty of Biology
Хат алмасуға жауапты Автор.
Email: avfilat@yandex.ru
Ресей, Moscow, 115522; Moscow, 119234