In vivo Proinflammatory Cytokine Production by CD-1 Mice in Response to Equipotential Doses of Rhodobacter capsulatus PG and Salmonella enterica Lipopolysaccharides
- Авторлар: Kabanov D.1, Rykov V.2, Prokhorenko S.3, Murashev A.2, Prokhorenko I.1
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Мекемелер:
- Institute of Basic Biological Problems
- Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
- Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitation
- Шығарылым: Том 83, № 7 (2018)
- Беттер: 846-854
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151690
- DOI: https://doi.org/10.1134/S0006297918070088
- ID: 151690
Дәйексөз келтіру
Аннотация
The capacities of relatively nontoxic lipopolysaccharide (LPS) from Rhodobacter capsulatus PG and highly potent LPS from Salmonella enterica serovar Typhimurium to evoke proinflammatory cytokine production have been compared in vivo. Intravenous administration of S. enterica LPS at a relatively low dose (1 mg/kg body weight) led to upregulation of TNF-α, IL-6, and IFN-γ production by non-sensitized CD-1 mice. LPS from R. capsulatus PG used at a four-times higher dose than that from S. enterica elicited production of almost the same amount of systemic TNF-α; therefore, the doses of 4 mg/kg LPS from R. capsulatus PG and 1 mg/kg LPS from S. enterica were considered to be approximately equipotential doses with respect to the LPS-dependent TNF-α production by CD-1 mice. Rhodobacter capsulatus PG LPS was a weaker inducer of the production of TNF-α, IL-6, and IFN-γ, as compared to the equipotential dose of S. enterica LPS. Administration of R. capsulatus PG LPS before S. enterica LPS decreased production of IFN-γ, but not of TNF-α and IL-6, induced by S. enterica LPS. Rhodobacter capsulatus PG LPS also suppressed IFN-γ production induced by S. enterica LPS when R. capsulatus PG LPS had been injected as little as 10 min after S. enterica LPS, but to a much lesser extent. Rhodobacter capsulatus PG LPS did not affect TNF-α and IL-6 production induced by the equipotential dose of S. enterica LPS. In order to draw conclusion on the endotoxic activity of particular LPSs, species-specific structure or arrangement of the animal or human immune systems should be considered.
Негізгі сөздер
Авторлар туралы
D. Kabanov
Institute of Basic Biological Problems
Хат алмасуға жауапты Автор.
Email: kabanovd1@rambler.ru
Ресей, Pushchino, Moscow Region, 142290
V. Rykov
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Email: kabanovd1@rambler.ru
Ресей, Pushchino, Moscow Region, 142290
S. Prokhorenko
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitation
Email: kabanovd1@rambler.ru
Ресей, Moscow, 107031
A. Murashev
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry
Email: kabanovd1@rambler.ru
Ресей, Pushchino, Moscow Region, 142290
I. Prokhorenko
Institute of Basic Biological Problems
Email: kabanovd1@rambler.ru
Ресей, Pushchino, Moscow Region, 142290