Effects of ouabain on proliferation of human endothelial cells correlate with Na+,K+-ATPase activity and intracellular ratio of Na+ and K+
- Авторлар: Tverskoi A.1, Sidorenko S.1, Klimanova E.1, Akimova O.1, Smolyaninova L.1, Lopina O.1, Orlov S.1,2,3
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Мекемелер:
- Faculty of Biology
- Tomsk National Research State University
- Siberian State Medical University
- Шығарылым: Том 81, № 8 (2016)
- Беттер: 876-883
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150981
- DOI: https://doi.org/10.1134/S0006297916080083
- ID: 150981
Дәйексөз келтіру
Аннотация
Side-by-side with inhibition of the Na+,K+-ATPase ouabain and other cardiotonic steroids (CTS) can affect cell functions by mechanisms other than regulation of the intracellular Na+ and K+ ratio ([Na+]i/[K+]i). Thus, we compared the doseand time-dependences of the effect of ouabain on intracellular [Na+]i/[K+]i ratio, Na+,K+-ATPase activity, and proliferation of human umbilical vein endothelial cells (HUVEC). Treatment of the cells with 1-3 nM ouabain for 24-72 h decreased the [Na+]i/[K+]i ratio and increased cell proliferation by 20-50%. We discovered that the same ouabain concentrations increased Na+,K+-ATPase activity by 25-30%, as measured by the rate of 86Rb+ influx. Higher ouabain concentrations inhibited Na+,K+-ATPase, increased [Na+]i/[K+]i ratio, suppressed cell growth, and caused cell death. When cells were treated with low ouabain concentrations for 48 or 72 h, a negative correlation between [Na+]i/[K+]i ratio and cell growth activation was observed. In cells treated with high ouabain concentrations for 24 h, the [Na+]i/[K+]i ratio correlated positively with proliferation inhibition. These data demonstrate that inhibition of HUVEC proliferation at high CTS concentrations correlates with dissipation of the Na+ and K+ concentration gradients, whereas cell growth stimulation by low CTS doses results from activation of Na+,K+-ATPase and decrease in the [Na+]i/[K+]i ratio.
Негізгі сөздер
Авторлар туралы
A. Tverskoi
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
S. Sidorenko
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
E. Klimanova
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
O. Akimova
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
L. Smolyaninova
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
O. Lopina
Faculty of Biology
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991
S. Orlov
Faculty of Biology; Tomsk National Research State University; Siberian State Medical University
Хат алмасуға жауапты Автор.
Email: sergeinorlov@yandex.ru
Ресей, Moscow, 119991; Tomsk, 634050; Tomsk, 634050