Binding of DNA with Abf2p increases efficiency of DNA uptake by isolated mitochondria
- Авторлар: Samoilova E.1, Krasheninnikov I.1, Vinogradova E.1, Kamenski P.1, Levitskii S.1
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Мекемелер:
- Faculty of Biology
- Шығарылым: Том 81, № 7 (2016)
- Беттер: 723-730
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150943
- DOI: https://doi.org/10.1134/S0006297916070087
- ID: 150943
Дәйексөз келтіру
Аннотация
Mutations in mitochondrial DNA often lead to severe hereditary diseases that are virtually resistant to symptomatic treatment. During the recent decades, many efforts were made to develop gene therapy approaches for treatment of such diseases using nucleic acid delivery into the organelles. The possibility of DNA import into mitochondria has been shown, but this process has low efficiency. In the present work, we demonstrate that the efficiency of DNA import can be significantly increased by preforming its complex with a mitochondria-targeted protein nonspecifically binding with DNA. As a model protein, we used the yeast protein Abf2p. In addition, we measured the length of the DNA site for binding this protein and the dissociation constant of the corresponding DNA–protein complex. Our data can serve as a basis for development of novel, highly efficient approaches for suppressing mutations in the mitochondrial genome.
Негізгі сөздер
Авторлар туралы
E. Samoilova
Faculty of Biology
Email: kamenski_pa@mail.bio.msu.ru
Ресей, Moscow, 119991
I. Krasheninnikov
Faculty of Biology
Email: kamenski_pa@mail.bio.msu.ru
Ресей, Moscow, 119991
E. Vinogradova
Faculty of Biology
Email: kamenski_pa@mail.bio.msu.ru
Ресей, Moscow, 119991
P. Kamenski
Faculty of Biology
Хат алмасуға жауапты Автор.
Email: kamenski_pa@mail.bio.msu.ru
Ресей, Moscow, 119991
S. Levitskii
Faculty of Biology
Email: kamenski_pa@mail.bio.msu.ru
Ресей, Moscow, 119991
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