Biotransformation of progesterone by the ascomycete Aspergillus niger N402
- Authors: Savinova O.S.1, Solyev P.N.2, Vasina D.V.1, Tyazhelova T.V.1, Fedorova T.V.1, Savinova T.S.3
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Affiliations:
- Bach Institute of Biochemistry, Biotechnology Research Center
- Engelhardt Institute of Molecular Biology
- Faculty of Chemistry
- Issue: Vol 83, No 1 (2018)
- Pages: 26-31
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151564
- DOI: https://doi.org/10.1134/S0006297918010030
- ID: 151564
Cite item
Abstract
The ability of the ascomyceteAspergillus niger N402 to transform exogenous progesterone was investigated. We found that this strain has steroid-hydroxylating activity and can introduce a hydroxyl group into the progesterone molecule mainly at positions C11(α) and C21 with predominant formation of 21-hydroxyprogesterone (deoxycortone). In addition, formation of 6β,11α-dihydroxyprogesterone was also observed. Studying the effects of the growth medium composition and temperature on progesterone conversion by A. niger N402 showed that the most intense accumulation of 21-hydroxyprogesterone occurred in minimal synthetic medium at 28°C. Increasing the cultivation temperature to 37°C resulted in almost complete inhibition of the hydroxylase activity in the minimal medium. In the complete medium, a similar increase in temperature inhibited 11α-hydroxylase activity and completely suppressed 6β-hydroxylase activity, but it produced no effect on 21-hydroxylating activity.
About the authors
O. S. Savinova
Bach Institute of Biochemistry, Biotechnology Research Center
Author for correspondence.
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119071
P. N. Solyev
Engelhardt Institute of Molecular Biology
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119991
D. V. Vasina
Bach Institute of Biochemistry, Biotechnology Research Center
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119071
T. V. Tyazhelova
Bach Institute of Biochemistry, Biotechnology Research Center
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119071
T. V. Fedorova
Bach Institute of Biochemistry, Biotechnology Research Center
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119071
T. S. Savinova
Faculty of Chemistry
Email: savinova_os@rambler.ru
Russian Federation, Moscow, 119991