The power and limitations of influenza virus hemagglutinin assays
- Authors: Ustinov N.B.1, Zavyalova E.G.1, Smirnova I.G.1, Kopylov A.M.1
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Affiliations:
- Lomonosov Moscow State University
- Issue: Vol 82, No 11 (2017)
- Pages: 1234-1248
- Section: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151496
- DOI: https://doi.org/10.1134/S0006297917110025
- ID: 151496
Cite item
Abstract
Influenza virus hemagglutinins (HAs) are surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus internalization. Development of methods for the inhibition of these processes drives progress in the design of new antiviral drugs. The state of the isolated HA (i.e. combining tertiary structure and extent of oligomerization) is defined by multiple factors, like the HA source and purification method, posttranslational modifications, pH, etc. The HA state affects HA functional activity and significantly impacts the results of numerous HA assays. In this review, we analyze the power and limitations of currently used HA assays regarding the state of HA.
About the authors
N. B. Ustinov
Lomonosov Moscow State University
Email: zlenka2006@gmail.com
Russian Federation, Moscow, 119991
E. G. Zavyalova
Lomonosov Moscow State University
Author for correspondence.
Email: zlenka2006@gmail.com
Russian Federation, Moscow, 119991
I. G. Smirnova
Lomonosov Moscow State University
Email: zlenka2006@gmail.com
Russian Federation, Moscow, 119991
A. M. Kopylov
Lomonosov Moscow State University
Email: zlenka2006@gmail.com
Russian Federation, Moscow, 119991