Escherichia coli signal peptidase recognizes and cleaves archaeal signal sequence
- Authors: Muhammad M.A.1, Falak S.1, Rashid N.1, Gardner Q.A.1, Ahmad N.2, Imanaka T.3, Akhtar M.1,4
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Affiliations:
- University of the Punjab, School of Biological Sciences
- University of the Punjab, Institute of Agricultural Sciences
- Ritsumeikan University, The Research Organization of Science and Technology
- University of Southampton, School of Biological Sciences
- Issue: Vol 82, No 7 (2017)
- Pages: 821-825
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151434
- DOI: https://doi.org/10.1134/S0006297917070070
- ID: 151434
Cite item
Abstract
Tk1884, an open reading frame encoding α-amylase in Thermococcus kodakarensis, was cloned with the native signal sequence and expressed in Escherichia coli. Heterologous gene expression resulted in secretion of the recombinant protein to the extracellular culture medium. Extracellular α-amylase activity gradually increased after induction. Tk1884 was purified from the extracellular medium, and its molecular mass determined by electrospray ionization mass spectrometry indicated the cleavage of a few amino acids. The N-terminal amino acid sequence of the purified Tk1884 was determined, which revealed that the signal peptide was cleaved between Ala26 and Ala27 by E. coli signal peptidase. To the best of our knowledge, this is the first report describing an archaeal signal sequence recognized and cleaved by E. coli signal peptidase.
About the authors
Majida Atta Muhammad
University of the Punjab, School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590
Samia Falak
University of the Punjab, School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590
Naeem Rashid
University of the Punjab, School of Biological Sciences
Author for correspondence.
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590
Qurra-tul-Ann Afza Gardner
University of the Punjab, School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590
Nasir Ahmad
University of the Punjab, Institute of Agricultural Sciences
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590
Tadayuki Imanaka
Ritsumeikan University, The Research Organization of Science and Technology
Email: naeem.ff.sbs@pu.edu.pk
Japan, Kusatsu, Shiga, 525-8577
Muhammad Akhtar
University of the Punjab, School of Biological Sciences; University of Southampton, School of Biological Sciences
Email: naeem.ff.sbs@pu.edu.pk
Pakistan, Lahore, 54590; SO16 7PX, Southampton