Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid
- Authors: Kasimova A.A.1,2, Shneider M.M.3, Arbatsky N.P.1, Popova A.V.4,5, Shashkov A.S.1, Miroshnikov K.A.3, Balaji V.6, Biswas I.7, Knirel Y.A.1
-
Affiliations:
- Zelinsky Institute of Organic Chemistry
- Higher Chemical College of the Russian Academy of Sciences
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Moscow Institute of Physics and Technology
- State Research Center for Applied Microbiology and Biotechnology
- Department of Clinical Microbiology
- Department of Microbiology
- Issue: Vol 82, No 4 (2017)
- Pages: 483-489
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151347
- DOI: https://doi.org/10.1134/S0006297917040101
- ID: 151347
Cite item
Abstract
Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Galp-(1→6)-β-D-Galp-(1→3)-D-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Galp-(1→3)-DGalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.
About the authors
A. A. Kasimova
Zelinsky Institute of Organic Chemistry; Higher Chemical College of the Russian Academy of Sciences
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991; Moscow, 125047
M. M. Shneider
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119997
N. P. Arbatsky
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
A. V. Popova
Moscow Institute of Physics and Technology; State Research Center for Applied Microbiology and Biotechnology
Email: yknirel@gmail.com
Russian Federation, Dolgoprudny, Moscow Region, 141701; Obolensk, Moscow Region, 142279
A. S. Shashkov
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
K. A. Miroshnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119997
Veeraraghavan Balaji
Department of Clinical Microbiology
Email: yknirel@gmail.com
India, Tamil Nadu, 632004
Indranil Biswas
Department of Microbiology
Email: yknirel@gmail.com
United States, Kansas City, KS, 66160
Yu. A. Knirel
Zelinsky Institute of Organic Chemistry
Author for correspondence.
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
![](/img/style/loading.gif)