Features of gene expression of Bacillus pumilus metalloendopeptidase
- Authors: Rudakova N.L.1, Sabirova A.R.1, Balaban N.P.1, Tikhonova A.O.1, Sharipova M.R.1
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Affiliations:
- Kazan (Volga Region) Federal University
- Issue: Vol 81, No 8 (2016)
- Pages: 884-891
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150983
- DOI: https://doi.org/10.1134/S0006297916080095
- ID: 150983
Cite item
Abstract
Features of gene expression of the secreted Bacillus pumilus metalloendopeptidase belonging to the adamalysin/reprolysin family were investigated. In the regulatory region of the gene, we identified hypothetical binding sites for transcription factors CcpA and TnrA. We found that the expression of the metalloendopeptidase gene is controlled by mechanisms of carbon and nitrogen catabolite repression. In experiments involving nitrogen metabolism regulatory protein mutant strains, we found that the control of the metalloendopeptidase gene expression involves proteins of ammonium transport GlnK and AmtB interacting with the TnrA-regulator.
About the authors
N. L. Rudakova
Kazan (Volga Region) Federal University
Author for correspondence.
Email: natalialrudakova@mail.ru
Russian Federation, Kazan, 420008
A. R. Sabirova
Kazan (Volga Region) Federal University
Email: natalialrudakova@mail.ru
Russian Federation, Kazan, 420008
N. P. Balaban
Kazan (Volga Region) Federal University
Email: natalialrudakova@mail.ru
Russian Federation, Kazan, 420008
A. O. Tikhonova
Kazan (Volga Region) Federal University
Email: natalialrudakova@mail.ru
Russian Federation, Kazan, 420008
M. R. Sharipova
Kazan (Volga Region) Federal University
Email: natalialrudakova@mail.ru
Russian Federation, Kazan, 420008