Peptide Aβ(16-25) forms nanofilms in the process of its aggregation
- Authors: Selivanova O.M.1, Gorbunova E.Y.2, Mustaeva L.G.2, Grigorashvili E.I.1, Suvorina M.Y.1, Surin A.K.1,3, Galzitskaya O.V.1
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Affiliations:
- Institute of Protein Research
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- State Research Center for Applied Microbiology and Biotechnology
- Issue: Vol 81, No 7 (2016)
- Pages: 755-761
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/150954
- DOI: https://doi.org/10.1134/S0006297916070129
- ID: 150954
Cite item
Abstract
A method for the synthesis and high purification of fragments of Aβ(1-42) peptide has been elaborated. We have synthesized the amyloidogenic fragment Aβ(16-25) predicted by us and studied the process of its aggregation by electron microscopy and X-ray analysis. Electron microscopy images show that the peptide forms a film, which is not characteristic of amyloid fibrils. At the same time, according to the X-ray diffraction data, its preparations display the presence of two main reflections (4.6-4.8 and 8-12 Å) characteristic of cross-β structure of amyloid fibrils. Thus, the fragment Aβ(16-25) that we predicted is a promising object not only for studying the process of polymerization of the peptides/proteins, but also for using it as a nanomaterial to study a number of biological processes.
About the authors
O. M. Selivanova
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290
E. Yu. Gorbunova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290
L. G. Mustaeva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290
E. I. Grigorashvili
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290
M. Yu. Suvorina
Institute of Protein Research
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290
A. K. Surin
Institute of Protein Research; State Research Center for Applied Microbiology and Biotechnology
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290; Obolensk, Moscow Region, 142279
O. V. Galzitskaya
Institute of Protein Research
Author for correspondence.
Email: ogalzit@vega.protres.ru
Russian Federation, Pushchino, Moscow Region, 142290