Main Strategies of Plant Expression System Glycoengineering for Producing Humanized Recombinant Pharmaceutical Proteins
- Authors: Rozov S.M.1,2, Permyakova N.V.1, Deineko E.V.1,3
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Affiliations:
- Federal Research Center Institute of Cytology and Genetics
- Novosibirsk State University
- National Tomsk State University
- Issue: Vol 83, No 3 (2018)
- Pages: 215-232
- Section: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151610
- DOI: https://doi.org/10.1134/S0006297918030033
- ID: 151610
Cite item
Abstract
Most the pharmaceutical proteins are derived not from their natural sources, rather their recombinant analogs are synthesized in various expression systems. Plant expression systems, unlike mammalian cell cultures, combine simplicity and low cost of procaryotic systems and the ability for posttranslational modifications inherent in eucaryotes. More than 50% of all human proteins and more than 40% of the currently used pharmaceutical proteins are glycosylated, that is, they are glycoproteins, and their biological activity, pharmacodynamics, and immunogenicity depend on the correct glycosylation pattern. This review examines in detail the similarities and differences between N- and O–glycosylation in plant and mammalian cells, as well as the effect of plant glycans on the activity, pharmacokinetics, immunity, and intensity of biosynthesis of pharmaceutical proteins. The main current strategies of glycoengineering of plant expression systems aimed at obtaining fully humanized proteins for pharmaceutical application are summarized.
About the authors
S. M. Rozov
Federal Research Center Institute of Cytology and Genetics; Novosibirsk State University
Author for correspondence.
Email: rozov@bionet.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
N. V. Permyakova
Federal Research Center Institute of Cytology and Genetics
Email: rozov@bionet.nsc.ru
Russian Federation, Novosibirsk, 630090
E. V. Deineko
Federal Research Center Institute of Cytology and Genetics; National Tomsk State University
Email: rozov@bionet.nsc.ru
Russian Federation, Novosibirsk, 630090; Tomsk, 634050
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