Heterologous expression and isolation of influenza A virus nuclear export protein NEP
- Authors: Golovko A.O.1, Koroleva O.N.1, Drutsa V.L.2
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Affiliations:
- Lomonosov Moscow State University
- Belozersky Institute of Physico-Chemical Biology
- Issue: Vol 82, No 12 (2017)
- Pages: 1529-1537
- Section: Regular Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151542
- DOI: https://doi.org/10.1134/S0006297917120124
- ID: 151542
Cite item
Abstract
Influenza A virus nuclear export protein NEP (NS2, 14.4 kDa) plays a key role in various steps of the virus life cycle. Highly purified protein preparations are required for structural and functional studies. In this study, we designed a series of Escherichia coli plasmid constructs for highly efficient expression of the NEP gene under control of the constitutive trp promoter. An efficient method for extraction of NEP from inclusion bodies based on dodecyl sulfate treatment was developed. Preparations of purified NEP with either N-or C-terminal (His)6-tag were obtained using Ni-NTA agarose affinity chromatography with yield of more than 20 mg per liter of culture. According to CD data, the secondary structure of the proteins matched that of natural NEP. A high propensity of NEP to aggregate over a wide range of conditions was observed.
About the authors
A. O. Golovko
Lomonosov Moscow State University
Email: koroleva@genebee.msu.ru
Russian Federation, Moscow, 119991
O. N. Koroleva
Lomonosov Moscow State University
Author for correspondence.
Email: koroleva@genebee.msu.ru
Russian Federation, Moscow, 119991
V. L. Drutsa
Belozersky Institute of Physico-Chemical Biology
Email: koroleva@genebee.msu.ru
Russian Federation, Moscow, 11991