Variability of methylation profiles of CpG sites in microRNA genes in leukocytes and vascular tissues of patients with atherosclerosis
- Authors: Kucher A.N.1,2, Nazarenko M.S.1, Markov A.V.1, Koroleva I.A.1, Barbarash O.L.3
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Affiliations:
- Research Institute of Medical Genetics, Tomsk National Research Medical Center
- National Research Tomsk State University
- Research Institute for Complex Issues of Cardiovascular Diseases
- Issue: Vol 82, No 6 (2017)
- Pages: 698-706
- Section: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151406
- DOI: https://doi.org/10.1134/S0006297917060062
- ID: 151406
Cite item
Abstract
In this study, we for the first time described the variability of methylation levels of 71 CpG sites in microRNA genes in leukocytes and blood vessels (coronary artery atherosclerotic plaques, intact internal thoracic arteries, and great saphenous veins) in patients with atherosclerosis using the Infinium HumanMethylation27 BeadChip microarray. Most of the analyzed CpG sites were characterized by the low variability, and most of these low-variable sites were hypomethylated in all tissue samples. CpG sites in coronary artery atherosclerotic plaques and leukocytes were similar in their methylation status. The highest variability of CpG methylation levels between different tissues was found for the CpG sites of the MIR10B gene; the methylation levels of these sites in leukocytes and atherosclerotic arteries were lower than in intact blood vessels. We also found that several cardiovascular disease risk factors, as well as medications, might affect methylation levels of CpG sites in microRNAs.
Keywords
About the authors
A. N. Kucher
Research Institute of Medical Genetics, Tomsk National Research Medical Center; National Research Tomsk State University
Author for correspondence.
Email: aksana.kucher@medgenetics.ru
Russian Federation, Tomsk, 634050; Tomsk, 634050
M. S. Nazarenko
Research Institute of Medical Genetics, Tomsk National Research Medical Center
Email: aksana.kucher@medgenetics.ru
Russian Federation, Tomsk, 634050
A. V. Markov
Research Institute of Medical Genetics, Tomsk National Research Medical Center
Email: aksana.kucher@medgenetics.ru
Russian Federation, Tomsk, 634050
I. A. Koroleva
Research Institute of Medical Genetics, Tomsk National Research Medical Center
Email: aksana.kucher@medgenetics.ru
Russian Federation, Tomsk, 634050
O. L. Barbarash
Research Institute for Complex Issues of Cardiovascular Diseases
Email: aksana.kucher@medgenetics.ru
Russian Federation, Kemerovo, 650002