Cytokine profile associated with selective removal of natural anti-αGal antibodies in a sepsis model in Gal-KO mice
- Authors: Cruz M.P.1, Gil D.B.1, Costa C.1, Mañez R.1,2
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Affiliations:
- Bellvitge Biomedical Research Institute (IDIBELL)
- Bellvitge University Hospital
- Issue: Vol 82, No 2 (2017)
- Pages: 205-212
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151263
- DOI: https://doi.org/10.1134/S0006297917020122
- ID: 151263
Cite item
Abstract
Selective depletion of natural anti-Galα1-3Galβ1-4GlcNAc (so-called anti-αGal) antibodies is achieved in α1,3-galactosyltransferase knockout (Gal-KO) mice by administration of the soluble glycoconjugate of αGal GAS914. This molecule removed up to 90% of natural circulating anti-αGal antibodies without causing unspecific production of cytokines in wild-type (CBA) and Gal-KO mice. However, the removal of anti-αGal antibodies in Gal-KO mice with GAS914 in the context of sepsis after cecal ligation and puncture (CLP) was associated with a significant increase in the production of leptin, CXLC1, CXLC13, and TIMP-1 cytokines compared to vehicle (PBS)-treated controls. Despite the current lack of understanding of the underlying mechanism, our data suggest a putative role of natural anti-αGal antibodies in the regulation of some cytokines during sepsis.
Keywords
About the authors
Magdiel Pérez Cruz
Bellvitge Biomedical Research Institute (IDIBELL)
Email: rmanez@bellvitgehospital.cat
Spain, Gran Via de l’Hospitalet 199, Hospitalet de Llobregat, 08908
Daniel Bello Gil
Bellvitge Biomedical Research Institute (IDIBELL)
Email: rmanez@bellvitgehospital.cat
Spain, Gran Via de l’Hospitalet 199, Hospitalet de Llobregat, 08908
Cristina Costa
Bellvitge Biomedical Research Institute (IDIBELL)
Email: rmanez@bellvitgehospital.cat
Spain, Gran Via de l’Hospitalet 199, Hospitalet de Llobregat, 08908
Rafael Mañez
Bellvitge Biomedical Research Institute (IDIBELL); Bellvitge University Hospital
Author for correspondence.
Email: rmanez@bellvitgehospital.cat
Spain, Gran Via de l’Hospitalet 199, Hospitalet de Llobregat, 08908; Feixa Llarga s/n, Hospitalet de Llobregat, 08907