Study of immunomodulatory effects of extracellular HSP70 in a mouse model of allergic airway inflammation
- Authors: Shevchenko M.A.1, Troyanova N.I.1, Servuli E.A.1, Bolkhovitina E.L.1, Fedorina A.S.1, Sapozhnikov A.M.1,2
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Biology
- Issue: Vol 81, No 11 (2016)
- Pages: 1384-1395
- Section: Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
- URL: https://journals.rcsi.science/0006-2979/article/view/151113
- DOI: https://doi.org/10.1134/S0006297916110158
- ID: 151113
Cite item
Abstract
Immunostimulatory properties of extracellular heat shock proteins 70 kDa (HSP70) became interesting for investigators a long time ago. However, in recent years a series of works showing a significant relation of the immunostimulating effects of recombinant HSP70 to contamination of the protein samples with bacterial endotoxins (lipopolysaccharide, LPS) has been published. The authors showed that intensive elimination of LPS from the protein samples resulted in inversion of immunostimulating effects of HSP70 to immunosuppressive activity of the protein. Nevertheless, at present the conception of immunostimulating, proinflammatory action of extracellular HSP70 is the most common. In this work, we studied immunomodulatory effects of exogenous HSP70 in a mouse model of allergic inflammation of airways. We also analyzed the dynamics of the level of the extracellular pool of HSP70 in the site of inflammation. The results demonstrated a considerable content of extracellular HSP70 in bronchoalveolar lavages with dynamics reflecting the stages of development of the induced inflammation. Oropharyngeal injection of exogenous HSP70 in the acute phase of allergic inflammation of airways resulted in significant suppression of the inflammatory process, which conforms to published data demonstrating an immunosuppressive activity of the extracellular pool of HSP70.
About the authors
M. A. Shevchenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997
N. I. Troyanova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997
E. A. Servuli
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997
E. L. Bolkhovitina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997
A. S. Fedorina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997
A. M. Sapozhnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology
Author for correspondence.
Email: amsap@mx.ibch.ru
Russian Federation, Moscow, 117997; Moscow, 119991