35S promoter methylation in kanamycin-resistant kalanchoe (Kalanchoe pinnata L.) plants expressing the antimicrobial peptide cecropin P1 transgene
- Authors: Shevchuk T.V.1, Zakharchenko N.S.1, Tarlachkov S.V.1, Furs O.V.1, Dyachenko O.V.1, Buryanov Y.I.1
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Affiliations:
- Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 81, No 9 (2016)
- Pages: 968-971
- Section: Accelerated Publication
- URL: https://journals.rcsi.science/0006-2979/article/view/151001
- DOI: https://doi.org/10.1134/S0006297916090054
- ID: 151001
Cite item
Abstract
Transgenic kalanchoe plants (Kalanchoe pinnata L.) expressing the antimicrobial peptide cecropin P1 gene (cecP1) under the control of the 35S cauliflower mosaic virus 35S RNA promoter and the selective neomycin phosphotransferase II (nptII) gene under the control of the nopaline synthase gene promoter were studied. The 35S promoter methylation and the cecropin P1 biosynthesis levels were compared in plants growing on media with and without kanamycin. The low level of active 35S promoter methylation further decreases upon cultivation on kanamycin-containing medium, while cecropin P1 synthesis increases.
About the authors
T. V. Shevchuk
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
N. S. Zakharchenko
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
S. V. Tarlachkov
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
O. V. Furs
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
O. V. Dyachenko
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
Y. I. Buryanov
Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: shevchuk@bibch.ru
Russian Federation, Pushchino, Moscow Region, 142290
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