Study of the Physicochemical and Biological Properties of a Protein–Peptide Complex Isolated from Cattle Ovarian Tissue

Resumo

A protein–peptide complex isolated from cattle ovarian tissues was studied. It was shown that it contains polypeptides with molecular weights from 800 to 6000 Da and a protein of the mammalian albumin family with a molecular weight of 66 690 Da containing the 16-member N-terminal amino acid sequence DTHKSEIAHRFKDLGE. The use of a model of the roller organotypic cultivation of rat ovaries demonstrated that the effect of this protein–peptide complex at low total protein concentrations of 10^–8–10^–15 mg/mL contributes to increases in the viability and maturation of secondary follicles in rat ovarian tissues in vitro.