Agrobacterium-Mediated Transformation of Lemna minor L. with Hirudin and β-Glucuronidase Genes


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

The Agrobacterium-mediated transformation of a duckweed plant (Lemna minor L.) with the use of the organogenic callus and nucleotide sequence of a gene for hirudin-1 optimized for expression in plants has been performed. Eight transgenic plant lines transformed by hirudin and seven lines transformed by the gene for β-glucuronidase were obtained. The expression of the glucuronidase gene was proven by histochemical staining and Western blot. ELISA of the transgenic plants showed that the content of β-glucuronidase in them varied from 0.28 to 1.43% of the total soluble protein. The expression of the hirudin-1 gene was confirmed by RT-PCR, with the maximum hirudin accumulation being equal to 0.02% of the total soluble protein. The results of the study can be used in the development of an expression system using the duckweed plant to obtain hirudin and other recombinant proteins for pharmaceutical use.

作者简介

O. Kozlov

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

T. Mitiouchkina

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

I. Tarasenko

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

L. Shaloiko

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

A. Firsov

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

编辑信件的主要联系方式.
Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

S. Dolgov

Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch

Email: aleksey_firsov@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290

补充文件

附件文件
动作
1. JATS XML

版权所有 © Pleiades Publishing, Inc., 2019