Flocculating Protein Flo1p from Saccharomyces cerevisiae W303-1A

Resumo

In Saccharomyces cerevisiae, flocculation is mainly regulated by the expression of genes belonging to the FLO family. In this study, the flocculating protein Flo1p, which is encoded by FLO1 gene was isolated and purified from S. cerevisiae W303-1A F4. The protein was found to have a molecular weight of about 200 kDa and belongs to Flo1-type. In addition, the recombinant yeasts including S. cerevisiae ZWA46 (Δ196-240), S. cerevisiae ZWA46 (Δ196-209) and S. cerevisiae ZWA46 (Δ210-240), were constructed. The results showed that amino acid residues from 196 to 209 of Flo1p were the active mannose-binding region, while residues 210 to 240 were not. The latter, however, had a certain influence on mannose-binding ability of Flo1p.