Achievements, Challenges, and Prospects in the Production of Nontransgenic, Genome-Edited Plants


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Abstract

The review summarizes the problems, achievements, and prospects of various approaches related to the delivery of components of DNA editing systems to plant cells, the regeneration of whole plants with an edited genome, and the development of transgene-free or transgene-clean crops. Here, an attempt was made to systematize the results of various studies describing the successful production of genome-edited (GE) plants with various genome modifications/mutations via the application of nuclease DNA–editing systems (ZFN, TALEN, and CRISPR/Cas). We discuss the main directions for the development of nuclease-based genome-editing methods to obtain GE plants free of foreign sequences from genome-editing tools.

About the authors

D. N. Miroshnichenko

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd

Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880

O. A. Shulga

All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences

Email: dolgov@bibch.ru
Russian Federation, Moscow, 127550

V. R. Timerbaev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences

Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550

S. V. Dolgov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd

Author for correspondence.
Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880

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