Achievements, Challenges, and Prospects in the Production of Nontransgenic, Genome-Edited Plants
- Authors: Miroshnichenko D.N.1,2,3, Shulga O.A.2, Timerbaev V.R.1,2, Dolgov S.V.1,2,3
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch
- All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
- Doka-Gene Technology Ltd
- Issue: Vol 55, No 9 (2019)
- Pages: 825-845
- Section: Problems and Prospects
- URL: https://journals.rcsi.science/0003-6838/article/view/153116
- DOI: https://doi.org/10.1134/S0003683819090047
- ID: 153116
Cite item
Abstract
The review summarizes the problems, achievements, and prospects of various approaches related to the delivery of components of DNA editing systems to plant cells, the regeneration of whole plants with an edited genome, and the development of transgene-free or transgene-clean crops. Here, an attempt was made to systematize the results of various studies describing the successful production of genome-edited (GE) plants with various genome modifications/mutations via the application of nuclease DNA–editing systems (ZFN, TALEN, and CRISPR/Cas). We discuss the main directions for the development of nuclease-based genome-editing methods to obtain GE plants free of foreign sequences from genome-editing tools.
About the authors
D. N. Miroshnichenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd
Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880
O. A. Shulga
All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
Email: dolgov@bibch.ru
Russian Federation, Moscow, 127550
V. R. Timerbaev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550
S. V. Dolgov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd
Author for correspondence.
Email: dolgov@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880
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