Highly Sensitive Immunochromatographic Assay for Qualitative and Quantitative Control of Beta-Agonist Ractopamine in Foods


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Abstract

An immunochromatographic method has been developed for the determination of ractopamine, a low-molecular, nonsteroidal growth regulator controlled in food due to toxicity. The developed analysis was characterized by an instrument detection limit of 0.05 ng/mL, a working range of 0.07‒0.28 ng/mL, and a visual detection limit of 0.5 ng/mL, which meets the requirements of sanitary and hygienic control. It is shown that the developed immunochromatographic test systems can be used to test meat products.

About the authors

E. A. Zvereva

Bach Institute of Biochemistry, Research Center of Biotechnology

Email: dzantiev@inbi.ras.ru
Russian Federation, Moscow, 119071

N. A. Shpakova

Bach Institute of Biochemistry, Research Center of Biotechnology

Email: dzantiev@inbi.ras.ru
Russian Federation, Moscow, 119071

A. V. Zherdev

Bach Institute of Biochemistry, Research Center of Biotechnology

Email: dzantiev@inbi.ras.ru
Russian Federation, Moscow, 119071

C. Xu

School of Food Science and Technology

Email: dzantiev@inbi.ras.ru
China, Wuxi, Jiangsu, 214122

B. B. Dzantiev

Bach Institute of Biochemistry, Research Center of Biotechnology

Author for correspondence.
Email: dzantiev@inbi.ras.ru
Russian Federation, Moscow, 119071

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