The Participation of calcium ions and reactive oxygen species in the induction of antioxidant enzymes and heat resistance in plant cells by hydrogen sulfide donor
- Authors: Kolupaev Y.E.1, Firsova E.N.1, Yastreb T.O.1, Lugovaya A.A.1
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Affiliations:
- Dokuchaev Kharkov National Agrarian University
- Issue: Vol 53, No 5 (2017)
- Pages: 573-579
- Section: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152312
- DOI: https://doi.org/10.1134/S0003683817050088
- ID: 152312
Cite item
Abstract
The effect of hydrogen sulfide (H2S) donor sodium hydrosulfide (NaHS) on the heat resistance of wheat (Triticum aestivum L.) coleoptile cells, the formation of reactive oxygen species (ROS), and the activity of the antioxidant enzymes in them was investigated. The treatment of coleoptiles with 100 µM NaHS caused transient enhancement of the generation of the superoxide anion radical (O2•) and an increased hydrogen peroxide content. The activities of antioxidant enzymes—superoxide dismutase, catalase, and guaiacol peroxidase— and coleoptile resistance to damaging heat was later found to have increased. The biochemical and physiological effects of the hydrogen sulfide donor described above were inhibited by the treatment of wheat coleoptiles with the hydrogen peroxide scavenger dimethylthiourea, the NADPH oxidase inhibitor imidazole, the extracellular calcium chelator EGTA, and the phosphatidylinositol-specific phospholipase C inhibitor neomycin. A conclusion was made on the role of ROS generation, which is dependent on the activity of NADPH oxidase and calcium homeostasis, in the transduction of the H2S signal, which induces antioxidant enzymes and the development of plant cell heat resistance.
About the authors
Yu. E. Kolupaev
Dokuchaev Kharkov National Agrarian University
Author for correspondence.
Email: plant.biology.knau@gmail.com
Ukraine, Kharkov, 62483
E. N. Firsova
Dokuchaev Kharkov National Agrarian University
Email: plant.biology.knau@gmail.com
Ukraine, Kharkov, 62483
T. O. Yastreb
Dokuchaev Kharkov National Agrarian University
Email: plant.biology.knau@gmail.com
Ukraine, Kharkov, 62483
A. A. Lugovaya
Dokuchaev Kharkov National Agrarian University
Email: plant.biology.knau@gmail.com
Ukraine, Kharkov, 62483
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