Synthesis of high-molecular-mass polyhydroxybutyrate from methanol in Methyloligella halotolerans C2


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Abstract

The influence of the concentrations of carbon and energy sources (methanol) and nitrogen (NH4+) on the yield and molecular mass (Mm) of poly-β-hydroxybutyrate (PHB) synthesized by the novel methylotroph Methyloligella halotolerans C2 was investigated. It was shown that the maximum concentrations of NH4+ and methanol in the medium for PHB biosynthesis were 0.15 g/L and 1 ± 0.2 mL/L, respectively. A unique high-molecular (8000–10000 kDa) PHB was obtained with NH4+ and methanol concentrations of 0.12 g/L and 1.25 mL/L, respectively, in a fermenter. The main physicochemical and mechanical properties of the obtained bioplastic with a molecular mass of 10000 kDa were determined.

About the authors

V. A. Ezhov

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290

N. V. Doronina

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290

M. N. Shmareva

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290

Yu. A. Trotsenko

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Author for correspondence.
Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290

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