Synthesis of high-molecular-mass polyhydroxybutyrate from methanol in Methyloligella halotolerans C2
- Authors: Ezhov V.A.1, Doronina N.V.1, Shmareva M.N.1, Trotsenko Y.A.1
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Affiliations:
- Skryabin Institute of Biochemistry and Physiology of Microorganisms
- Issue: Vol 53, No 1 (2017)
- Pages: 47-51
- Section: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152146
- DOI: https://doi.org/10.1134/S0003683817010112
- ID: 152146
Cite item
Abstract
The influence of the concentrations of carbon and energy sources (methanol) and nitrogen (NH4+) on the yield and molecular mass (Mm) of poly-β-hydroxybutyrate (PHB) synthesized by the novel methylotroph Methyloligella halotolerans C2 was investigated. It was shown that the maximum concentrations of NH4+ and methanol in the medium for PHB biosynthesis were 0.15 g/L and 1 ± 0.2 mL/L, respectively. A unique high-molecular (8000–10000 kDa) PHB was obtained with NH4+ and methanol concentrations of 0.12 g/L and 1.25 mL/L, respectively, in a fermenter. The main physicochemical and mechanical properties of the obtained bioplastic with a molecular mass of 10000 kDa were determined.
Keywords
About the authors
V. A. Ezhov
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
N. V. Doronina
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
M. N. Shmareva
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
Yu. A. Trotsenko
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Author for correspondence.
Email: trotsenko@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
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